| 摘要: |
| [目的] 以槐耳菌丝体生物量及多糖为主要指标,探究槐耳-甘草液体共发酵中最佳甘草的添加量和发酵时长。[方法] 采用液体发酵技术,在液体发酵培养基中加入甘草提取液,共发酵获得槐耳-甘草共发酵产物,苯酚-硫酸法及DNS法结合测定多糖含量。[结果] 槐耳-甘草液体共发酵实验中,添加甘草8 g/L、发酵终点设为192 h时,槐耳生物量和胞内多糖产量达到最大值。[结论] 运用槐耳-甘草液体共发酵可以提高槐耳生物量及多糖的含量,为深入研究槐耳-甘草液体共发酵机制提供了理论依据。 |
| 关键词: 槐耳 液体发酵 多糖 |
| DOI：10.11656/j.issn.1672-1519.2015.09.14 |
| 分类号: |
| 基金项目:国家科技基础性工作项目(2007FY110600);国家自然科学基金项目资助(810001632);国家自然基金面上项目资助(81374050)。 |
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| Preliminary study on the impact of Glycyrrhizae Radix on the growth of Trametes robiniophila mycelium and polysaccharide in liquid fermentation |
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FENG Shan, LI Sai-nan, JIANG Lin-qian, CHANG Yan-xu, MA Lin
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Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To explore the best addition amount of Glycyrrhizae Radix and fermentation time of the liquid fermentation of Trametes robiniophila-Glycyrrhizae Radix, with mycelial biomass and fungi polysaccharide as the main indicators. [Methods] Mycelial of Trametes robiniophila was obtained in liquid fermentation, with Glycyrrhizae Radix added to liquid fermentation medium. The content of polysaccharides was determined by phenol-sulfuric acid method and DNS method. [Results] The best addition amount of Glycyrrhizae Radix was 8 g/L and the best fermentation time was 192 h when the biomass and polysaccharide of Trametes robiniophila got to the maximum. [Conclusion] Using Trametes robiniophila-Glycyrrhizae Radix liquid fermentation could increase the mycelial biomass and the content of intracellular and extracellular polysaccharides of Trametes robiniophila. It provides a theoretical basis for improving the yield of liquid fermentation. |
| Key words: Trametes robiniophila liquid fermentation polysaccharide |
