| 摘要: |
| [目的] 建立知母药材的高效液相色谱仪-二极管阵列检测器-蒸发光散射检测器联用(HPLC/PDA/ELSD)指纹图谱。[方法] 采用Waters Symmetry ShieldTM RP C18(5 μm,4.6 mm×250 mm )色谱柱,流动相为乙腈-0.1%冰醋酸,梯度洗脱流速1.0 mL/min,柱温30 ℃,检测波长258 nm,ELSD检测器载气气压30 Psi,漂移管温度80 ℃。[结果] 建立了知母的HPLC/PDA/ELSD指纹图谱,在HPLC-PDA色谱图中标定了17个共有峰,HPLC-ELSD色谱图中标定了14个共有峰。[结论] 该方法重复性好,可用于知母药材的全面质量控制。 |
| 关键词: 知母 指纹图谱 HPLC/PDA/ELSD |
| DOI：10.11656/j.issn.1672-1519.2015.10.10 |
| 分类号: |
| 基金项目:“十二五”科技部国家科技支撑计划项目(2011BA107B064)。 |
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| Study on HPLC/PDA/ELSD fingerprint of Rhizoma Anemarrhenae |
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WEI Jing-na1, LIU Zheng-hui1, ZHAO Lin-lin1, GUO Yong-ze2, CHENG Yi2, WEN Chun-xiu3
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1.Tianjin High-Standard Quality Testing Lab, Tianjin 300381, China;2.Tianjin Agricultural Quality Standards and Testing Technology Research Institute, Tianjin 300381, China;3.Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Science, Shijiazhuang 050051, China
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| Abstract: |
| [Objective] To establish the HPLC/PDA/ELSD fingerprint of Rhizoma Anemarrhenae. [Methods] This experiment had been done on Waters Symmetry ShieldTM RP C18 (5 μm,4.6 mm×250 mm) analytical column of 30 ℃, with a gradient eluted acetonitrile-0.1% acetic acid mobile phase system of 1.0 mL/min. The detection wavelength had been set at 258 nm, N2 pressure was 30 Psi, and the temperature of the drift tube was 80 ℃. [Results] The mutual modes of the HPLC/PDA/ELSD fingerprint were set up under the established condition. The 17 mutual peaks in the HPLC-PDA fingerprint and 14 mutual peaks in the HPLC-ELSD fingerprint of 11 samples were confirmed. [Conclusion] The method that had been established in this experiment is a good method to totally control the quality of Rhizoma Anemarrhenae. |
| Key words: Rhizoma Anemarrhenae fingerprint HPLC/PDA/ELSD |
