| 摘要: |
| [目的] 拟用β-环糊精为促渗剂提高丹酚酸B透过肠黏膜的能力,以及考察不同质量浓度β-环糊精对丹酚酸B肠黏膜透过性的促进作用。[方法] 选择大鼠的十二指肠、空肠、回肠、结肠为考察肠段,用Ussing chamber技术考察丹酚酸B溶液及添加β-环糊精促渗剂的丹酚酸B溶液分别在4个肠段的透过情况。[结果] β-环糊精能明显提高丹酚酸B在十二指肠、空肠、回肠3个肠段的透过性;180 min后β-环糊精组的肠段的电阻降为初始电阻值的65%;质量浓度为0.13%的β-环糊精促渗效果优于质量浓度0.65%、1.3%的β-环糊精的促渗效果。[结论] β-环糊精能够提高丹酚酸B透过肠黏膜的能力,这可能与β-环糊精能够打开细胞间紧密连接有关系;当β-环糊精的浓度从0.13%升到1.3%,丹酚酸B的表观渗透系数随着浓度升高而减小,其机制有待进一步研究。 |
| 关键词: 丹酚酸B 尤斯灌流池 肠吸收 促渗剂 |
| DOI：10.11656/j.issn.1672-1519.2016.01.12 |
| 分类号: |
| 基金项目:新世纪优秀人才支持计划(NCET-12-1068)。 |
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| Study on the effects of beta cyclodextrin on improving the permeability of salvianolic acid B passing through intestinal mucosa |
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LIU-Xiao lei1, ZHANG Li1, LIU-Zhi dong1, LIU Yang2, HU-Li min1, ZHANG-Wei3
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1.Institute of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;3.First People's Hospital in Wu'an, Wu'an 056300, China
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| Abstract: |
| [Objective] β-cyclodextrin was acted as enhancers to improve the permeability ability of salvianolic acid B passing through the intestinal mucosa. The study also discussed that the penetration enhancing effect of different concentrations of β-cyclodextrin on intestinal mucosal permeability of salvianolic acid B. [Methods] By Ussing chamber technology, duodenum, jejunum, ileum and colon of rats were selected for studying the permeability of salvianolic acid B solution and the addition of β-cyclodextrin in salvianolic acid B solution. [Results] The results showed that: β-cyclodextrin could improve the permeability of salvianolic acid B in duodenum, jejunum and ileum significantly. In 180 min, the resistance of intestine in β-cyclodextrin group was reduced to 65% of the initial resistance value. The promoting effect of 0.13% mass concentration of β-cyclodextrin was better than that of 0.65% and 1.3% mass concentration of β-cyclodextrin. [Conclusion] β-cyclodextrin could improve the permeability of salvianolic acid B passing through the intestinal mucosa, which might be associated with the effect of β-cyclodextrin on opening of the intercellular tight junction proteins. When the concentration of β-cyclodextrin increased from 0.13% to 1.3%, the apparent permeability coefficient of salvianolic acid B decreased along with the increasing of β-cyclodextrin concentration. The mechanism would have to be further studied. |
| Key words: salvianolic acid B ussing chamber intestinal absorption penetration enhancer |
