| 摘要: |
| [目的] 观察大株红景天注射液抗心肌缺氧/复氧损伤的保护作用,并探讨其抗心肌缺氧/复氧可能的机制。[方法] 原代培养的心肌细胞随机分为5组:空白对照组;缺氧/复氧组;缺氧/复氧分别加大株红景天注射液5、10、20 mL/L组。加药孵育12 h后,缺氧培养9 h,随后常氧培养2 h.噻唑蓝(MTT)法检测细胞增殖,CMH2DCF-DA荧光探针检测细胞内活性氧簇(ROS)的生成量,JC-1荧光探针检测细胞内线粒体膜电位,以及测定细胞内超氧化物歧化酶SOD活力及其基因表达量。[结果] 大株红景天注射液增加了缺氧/复氧损伤心肌细胞的活力,减少细胞内ROS的生成,抑制细胞内线粒体膜电位的降低,同时增加了心肌细胞内SOD的活力及其基因表达量。[结论] 大株红景天注射液对缺氧/复氧(H/R)损伤的心肌细胞有保护作用。 |
| 关键词: 大株红景天注射液 缺氧/复氧 原代心肌细胞 氧化应激 |
| DOI：10.11656/j.issn.1672-1519.2016.03.09 |
| 分类号: |
| 基金项目:国家重点基础研究发展计划资助项目(2012CB518404);国家自然基金资助项目(81273993). |
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| Protective effects and mechanisms of sofren injection on primary cardiomyocytes hypoxia-reoxygenation injury |
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WANG Ya-dong, Patrick Asare Fordjour, LI Lan, WEI Jing, FAN Guan-wei
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Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the effect of sofren injection on primary cardiomyocytes damaged by hypoxia-reoxygenation and to research signal pathways involved in the mechanism of sofren injection. [Methods] The cell viability was measured by MTT. Primarily cultured cardiomyocytes were pretreated with different concentrations of sofren injection, cultured in hypoxia for 9 h and reoxygenation for 2 h. The cell viability was measured by MTT. The ROS generation and mitochondrial membrane potential in the cell were measured by CMH2DCF-DA and JC-1 fluorescent probe, the vitality and gene expression of superoxide dismutase (SOD). [Results] Compared with hypoxia-reoxygenation group, pretreated with different concentrations of sofren injection significantly attenuated model-induced cell viability and mitochondrial membrane potential loss, dramatically decreased the ROS generation. The SOD vitality and gene expression were increased by sofren injection. [Conclusion] Sofren injection can protect rat cardiomyocytes from the injury induced by hypoxia-reoxygenation. |
| Key words: sofren injection hypoxia-reoxygenation primary cardiomyocyte oxidative stress |
