一测多评法同时测定胃力康颗粒中7种成分的含量

Simultaneous determination of seven constituents in Wenlikang granules by quantitative analysis of multi-components by single marker

关键词:  胃力康颗粒  一测多评法  超高效液相色谱法  质量控制

Key words:  Weilikang granules  quantitative analysi of multi-components by single marker  UPLC  quality control

摘要:

[目的] 建立可用于胃力康颗粒中7种成分含量测定的一测多评法。[方法] 采用超高效液相色谱(UPLC),以芍药苷为内参物,确定芦丁、柚皮苷、迷迭香酸、丹酚酸B、大黄素-8-O-β-D-葡萄糖苷、甘草酸的相对校正因子,通过相对校正因子计算各成分的含量;同时比较10批胃力康颗粒一测多评法的计算值与外标法测定结果的差异。[结果] 建立了胃力康颗粒中7种成分一测多评的含量测定方法,10批胃力康颗粒中7种成分采用一测多评法测定与外标法测定结果无明显差异。[结论] 本实验建立的一测多评法可用于胃力康颗粒中7种成分的含量测定,方法快速、准确,可用于胃力康颗粒的质量控制。

Abstract:

[Objective] To establish a method for simultaneous determination of seven bioactive components in Weilikang Granules by quantitative analysis of multi-components by single marker (QAMS). [Methods] Peoniflorin was select as an index, relative correction factors of Rutin, Naringin, Rosmarinic acid, Salvianolic acid B, Emodin-8-O-β-D-glucopyranoside and Glycyrrhizic acid were established by UPLC and calculated to achieve QAMS. The accuracy of the new method was evaluated by comparing the results between QAMS and the external standard method. [Results] The analysis method was established and there is no significant difference between the calculated contents and the contents which were determined by external reference method in 10 batches of samples. [Conclusion] The QAMS method is reliable, accurate to control the quality of seven bioactive components of Weilikang granules.