桃核承气汤对糖尿病大鼠大血管纤维病变Toll样受体通路作用研究

Study of Taohe Chengqi Tang on Toll-like receptor passage in diabetic vascular fibrosis with rats

关键词:  大血管纤维化  桃核承气汤  Toll样受体2  Toll样受体-4

Key words:  macrovascular fibrosis  THCQT  Toll-like receptor-2  Toll-like receptor-4

摘要:

[目的] 通过免疫印记法检测实验性Ⅱ型糖尿病大鼠下肢股血管中Toll样受体-2(TLR-2)、Toll样受体-4(TLR-4)表达,并通过观察桃核承气汤对相关指标影响,揭示糖尿病大血管纤维化病变的发病机制,及中药桃核承气汤在糖尿病血管纤维化中的干预作用。[方法] 雄性SD大鼠170只,随机分为5组:A-正常对照组(n=30),余140只造糖尿病模型模,造模成功123只,随机分为4组:B-模型对照组(n=31)、C-中药高剂量治疗组(n=30)、D-中药中剂量治疗组(n=31)、E-中药低剂量治疗组(n=31); A组不予药物干预,B组每日给予0.9%生理盐水10 mL/kg灌胃,C组每日给予桃核承气汤1.8g/kg,D组每日给予桃核承气汤0.9g/kg,E组每日给予桃核承气汤0.45g/kg。药物干预第1周、12周、20周,按计划处死大鼠,各组分别取材,保存标本,免疫印记法检测TLR-2、TLR-4沉积部位及表达情况。[结果] TLR-2、TLR-4在糖尿病大鼠股动脉中均显著表达,中药桃核承气汤干预可有效降低TLR-2及TLR-4的表达。[结论] 糖尿病大血管病变可能与Toll样受体通路有关,中药组方桃核承气汤对糖尿病大血管病变有明显改善作用,可减缓纤维化进程,其干预作用于用药剂量和用药时间相关。

Abstract:

[Objective] Using Western blotting to detect the expression of TLR-2, TLR-4 which from the diabetes rats in lower limb femoral vessels, and observing by Taohe Chengqi Tang(THCQT) impact on related indicators, revealing intervention mechanisms of THCQT on diabetic vascular fibrosis. [Methods] The 170 male SD rats were divided into five groups, A- the normal control group (n=30), other 140 making diabetes model and succeed 123, randomly divided into 4 groups:B- model control group (n=31), C- Chinese high-dose treatment group (n=30), D-medicine dose treatment group (n=31), E- low dose treatment group (n=31). A group was no drug intervention, group B received 0.9% saline 10 mL/(kg·d) orally, group C was given THCQT 1.8 g/kg, D group was given THCQT 0.9 g/kg, E group was given THCQT 0.45 g/kg. Drug intervention first weeks, 12 weeks, 20 weeks, the rats were sacrificed according to plan, the groups were drawn, preserved specimens, Western blotting detection TLR-2 and TLR-4 expression. [Results] TLR-2 and TLR-4 has significant expression in diabetic rat femoral artery, TCD THCQT can effectively reduce the expression of TLR-2 and TLR-4. [Conclusion] Diabetic vascular disease may be associated with Toll-like receptor pathway and apoptosis, TCD THCQT can obviously improve diabetic vascular lesions, and slow down the fibrosis process, its intervention effect associated with dosage and duration.