参竹精片协同戊巴比妥钠对小鼠睡眠及5-HT的影响

Effects of Shenzhujing tablets for sodium pentobarbital-induced hypnosis in mice

关键词:  失眠  参竹精片  5-HT

Key words:  insomnia  Shenzhujing tablet  5-HT

摘要:

[目的] 观察参竹精片对睡眠的影响。[方法] 选昆明小鼠随机分为正常对照组、参竹精片0.5、0.25、0.125 g/kg组,分别单次灌胃给药和连续7 d给药,末次给药后2 h,各组小鼠分别以30 mg/kg(阈下剂量)和50 mg/kg(阈剂量)腹腔注射戊巴比妥钠,观察小鼠入睡时间、入睡率及睡眠时间,测定脑干组织5-羟色胺(5-HT)、5-羟基吲哚乙酸(5-HIAA)含量。[结果] 与正常组比较,单次灌胃给药和连续7 d给药,参竹精片可明显增加戊巴比妥钠(阈下剂量)致小鼠的入睡率(0.5 g/kg,P<0.05),减少戊巴比妥钠(阈剂量)致小鼠的入睡时间(P<0.01),延长睡眠时间(P<0.01),增加脑干组织5-HT含量(P<0.05)。[结论] 参竹精片可促进脑组织分泌5-HT,减少入睡时间、提高入睡率、延长睡眠时间,具有一定改善睡眠的作用。

Abstract:

[Objective] To observe the effects of Shenzhujing tablets on sodium pentobarbital-induced hypnosis. [Methods] The 96 of Kunming mice were randomly divided into 4 groups: normal group, and Shenzhujing tablet (0.5, 0.25, 0.125 g/kg) groups. The drugs were administrated orally in single doses to 12 of the mice in each group 2 h before the intraperitoneal injection of sodium pentobarbital at the dose of 30 mg/kg or 50 mg/kg, respectively. Then the mice received daily oral treatments of drugs for 7 days. Similarly, Sodium pentobarbital-induced hypnosis tests were also used 2 h after the last administration of the drugs. The sleep latency and sleep duration during pentobarbital-induced sleep were measured and the ratios of sleep mice to non-sleep mice were counted in mice after oral administration of the drugs. Moreover, the contents of the 5-hydroxytryptamine(5-HT) and 5-hydroxyindoleacetic acid(5-HIAA)in the brain stem were determined. [Results] Compared with normal group, Shenzhujing tablets significantly prolonged the pentobarbital-induced sleep duration and decrease sleep latency at dose of 0.5 g/kg(P<0.05), increase the ratios of sleep mice(P<0.01) to non-sleep mice and the contents of 5-HT in the brain stem under the condition of 30 mg/kg of sodium pentobarbital (P<0.05). [Conclusion] Shenzhujing tablets possess sedative-hypnotic activity possibly by promoting the secretion of 5-HT in the brain and might be effective agents for treatment of insomnia.