榛花消肾安胶囊干预实验性糖尿病大鼠足细胞相关蛋白琢-actinin-4的研究

Study on the podocyte associated protein α-actinin-4 of experimental diabetic rats treated with Zhenhua Xiaoshen An capsules

关键词:  榛花消肾安胶囊  糖尿病肾病  足细胞α-actinin

Key words:  Zhenhua Xiaoshen An capsule  diabetic nephropathy  podocyte  α-actinin

摘要:

[目的] 通过实验观察实验性糖尿病（DM）大鼠的肾脏及足细胞相关蛋白α-actinin-4的表达，表明“榛花消肾安胶囊”治疗糖尿病肾病的有效性。[方法] 建立链脲佐菌素（STZ）诱导的实验性糖尿病大鼠模型分为DM模型组、对照组、中药组、西药组，中药组给予榛花消肾安胶囊，西药组给予厄贝沙坦片；检测空腹血糖、24 h尿微量白蛋白、血肌酐（Scr）、尿素氮（BUN），应用光学显微镜和透射电镜及免疫组化方法观察足细胞骨架蛋白α-actinin-4的表达；应用实时定量聚合酶链反应（PCR）技术检测α-actinin-4 m RNA表达。[结果] 中药干预之后中药高、中、低剂量组及西药对照组尿量较DM模型组明显减少（P<0.01）；中药各剂量组与西药对照组大鼠血糖均明显低于DM模型组（P<0.01）；中药各剂量组和西药对照组大鼠α-actinin-4及mRNA含量均低于DM模型组（P<0.01）。[结论] 榛花消肾安胶囊能够降低实验性DM大鼠血糖、Scr、BUN，减少尿微量白蛋白的排泄，而改善肾功能；能够抑制实验性DM大鼠早期肾脏体积增大，而抑制α-actinin-4蛋白表达及其m RNA的表达。

Abstract:

[Objective] Through this experiment to observe the experimental diabetic rats renal and podocyte associated protein α-actinin-4 expression, indicate Zhenhua Xiaoshen An capsules effectiveness of diabetic nephropathy in the treatment of diabetic nephropathy.[Methods] To establish the model of STZ induced diabetic rats model group, control group, Traditional Chinese medicine group, Western medicine group. Traditional Chinese medicine group was given Zhenhua Xiaoshen An capsule. Western medicine group was given irbesartan. Fasting blood glucose, 24 h Urine micro albumin, Scr, BUN were detected with optical microscope, transmission electron microscope and immunohistochemistry in order to observe the expression of podocyte skeletal protein α-actinin-4. The expression of α-actinin-4 mRNA were detected with Real-time quantitative PCR.[Results] After the intervention, the urine volume of traditional Chinese Medicine traditional Chinese medicine high, middle, low dose group and Western medicine control group obviously decreased, compared with that of DM model group (P<0.01). The blood glucose of rats in Traditional Chinese medicine every dose group and Western medicine control group were obvious lower than that in DM model group (P<0.01). The α-actinin-4 mRNA contents of rats in traditional Chinese medicine every dose group and Western medicine control group were lower than that in DM model group (P<0.01).[Conclusion] Zhenhua Xiaoshen An capsules can reduce to experimental DM rats blood glucose, serum creatinine, blood urea nitrogen and excrete urine micro albumin, and improve renal function. It also can restrain the incresing of the renal volume of experimental DM rats at early stage and the protein and mRNA expression of α-actinin-4.