利用神经示踪技术初步探讨针刺人迎穴降血压的动脉压力反射机制

Preliminary researching on the antihypertensive mechanism of acupuncture Renying (ST9) point based on neural tracer technology

关键词:  人迎穴  针刺  高血压  神经示踪

Key words:  Renyin  acupuncture  hypertension  neural tracer technique

摘要:

[目的] 利用神经示踪技术，从动脉压力反射角度，初步探讨针刺人迎穴的降压机制。[方法] 选取SPF级雄性自发性高血压大鼠（SHR）45只，随机分为：人迎组、人迎加手法组、空白对照组，每组15只；并选取正常雄性Wistar大鼠15只，作为正常空白对照组。人迎组针刺人迎后留针30 min；人迎加手法组针刺人迎后行小幅度（小于90°）、高频率（每分钟120～160次）的捻转补法，施术1 min，留针30 min；其余两组不进行针刺，干预周期28天。分别于干预前、后测定血压；干预结束后在大鼠人迎穴注射PRV-152病毒，镜下观察其延髓及下丘脑平面染色情况。[结果] 人迎加手法组的降压效应明显优于其他组。各组大鼠下丘脑、延髓中PRV阳性细胞计数组间差异具有统计学意义（P<0.001），其中人迎加手法组PRV阳性细胞在下丘脑、延髓中计数明显高于其他组。[结论] 针刺人迎穴，可能通过增强相关轴浆运输通路的敏感性，来实现降压效应。

Abstract:

[Objective] Using neural tracer technology, from the perspective of arterial pressure reflection, preliminary research acupuncture Renying(ST9) point antihypertensive mechanism.[Methods] 45 SPF grade male 12 W SHR were selected and randomly divided into Renying group, Renying and manipulation group, blank control group, 15 rats in each group. 15 male normotensive Wistar rats were as normal blank control group. Renying group was acupunctured Renying point and stayed for 30minutes. Renying and manipulation group underwent small amplitude attack (less than 90 degrees) and high frequency (120~160 times/min) twisting as reinforcing method and operated for 1 minutes, stayed for 30minutes. Treatment lasted 28 days. Normotensive Wistar rats control group and SHR control group rats were without acupuncture. Blood pressure was measured before and after treatment. In the rats of Renying injection of PRV-152 virus, observed the medulla and hypothalamus under the plane mirror after intervention. [Results] The difference of hypothalamus and medulla oblongata PRV positive cell counts between two group was statistical significance (P<0.001). Acupuncture attack plus massage group PRV positive cells counts in the hypothalamus and medulla oblongata was significantly higher than other groups. [Conclusion] The antihypertensive effect may be achieved by enhancing the sensitivity of the transport pathway by acupuncture Renying point.