苦瓜-人参预防给药对高脂诱导肥胖小鼠胰岛素抵抗的影响

Effect of preventive administration of Momordica charantia-Panax ginseng herb on insulin resistance in high fat diet-induced obesity mice

关键词:  苦瓜  人参  苦瓜-人参对药  胰岛素抵抗  胰岛素敏感性  高脂诱导肥胖小鼠

Key words:  Momordica charantia  Panax ginseng  Momordica charantia-Panax ginseng herb couple  insulin resistance  insulin sensitivity  diet induced obesity mice

摘要:

[目的] 考察苦瓜、人参、苦瓜-人参预防给药对高脂诱导肥胖小鼠糖脂代谢及胰岛素抵抗的影响，为“一补一泄、一寒一热”苦瓜-人参对药改善肥胖、胰岛素抵抗，预防2型糖尿病提供实验证据。[方法] 采用高脂饲料喂养法诱导肥胖胰岛素抵抗小鼠模型，并给予苦瓜、人参、苦瓜-人参对药分别进行预防性干预，以二甲双胍作为阳性对照。给药干预16周后，测摄食量、体质量、脂肪指数、空腹血糖（FPG）、空腹血清胰岛素（FINS）、血脂水平，计算稳态模型胰岛素抵抗指数、胰岛素敏感指数，评价胰岛素抵抗与分泌水平，口服葡萄糖耐量实验考察血糖调节能力。苏木精-伊红（HE）染色观察睾周脂肪细胞形态。[结果] 高脂饲料诱导小鼠的体质量、皮下脂肪指数、FPG、FINS水平明显升高，并发生明显胰岛素抵抗，而人参可以显着改善胰岛素抵抗，苦瓜可明显降低FPG、体质量和皮下脂肪含量，苦瓜-人参对药则可明显降低FINS及皮下脂肪含量，并且各给药干预组在不同程度上改善了肥胖小鼠的脂肪细胞形态。[结论] 人参能明显改善肥胖小鼠胰岛素抵抗，苦瓜可以有效减轻其FPG及体质量，苦瓜-人参对药降低FINS更有优势。

Abstract:

[Objective] To investigate the effects of preventive administration of Momordica charantia, Panax ginseng and Momordica Charantia-Panax ginseng herb on glucolipid metabolism and insulin resistance in high-fat diet induced obesity mice, and providing experimental evidence for the herb couple improving obesity and insulin resistance, therefore prevent T2DM. [Methods] Using insulin resistance model of high fat induced obesity mice, and fed Momordica charantia, ginseng, the herb couple respectively, with metformin as positive control. The food intake and body weight were measured every week. After 16 weeks drug intervention, the fat index, fasting plasma glucose (FPG), fasting insulin (FINS) and blood lipid were investigated; the level of insulin resistance was estimated by homeostasis model assessment for insulin resistance (HOMA-IR) and insulin sensitivity index (ISI); the level of regulating blood sugar was observed by oral glucose tolerance test (OGTT); histopathological changes of the adipose tissue were observed by HE staining. [Results] Ginseng can significantly improve insulin resistance. Momordica charantia can obviously reduce the FPG, weight and subcutaneous fat. The herb couple can obviously reduce the FINS and subcutaneous fat. Above intervention groups improved fat cells' form of obese mice in different extent. [Conclusion] Ginseng can significantly improve insulin resistance in obesity mice, Momordica charantia can effectively reduce the FPG and weight, the herb couple have more advantages to reduce its FINS.