和颜坤泰胶囊对卵巢去势大鼠子宫形态及细胞凋亡的影响

Anti-apoptotic and uterotrophic effects of Heyan Kuntai capsules in uterus of ovariectomized rats

关键词:  和颜坤泰胶囊  子宫形态学  细胞凋亡  Bcl-2 mRNA和蛋白  Bax mRNA和蛋白

Key words:  Heyan Kuntai capsule  uterus morphology  apoptosis  Bcl-2 mRNA and protein  Bax mRNA and protein

摘要:

[目的]研究和颜坤泰胶囊对卵巢去势模型大鼠子宫形态及细胞凋亡的影响。[方法]以雌性去势大鼠为动物模型，分为空白对照组，假手术组，模型组，补佳乐组，莉芙敏组，和颜坤泰胶囊8、4、2 g生药/kg剂量组。给药8周后，观察药物对子宫病理学的影响，检测子宫细胞凋亡率及Bcl-2 mRNA、Bax mRNA和蛋白的表达。[结果]和颜坤泰胶囊可增加去势大鼠子宫横截面及壁厚，肌层面积及厚度，内膜面积及厚度，上皮厚度和腺体数（P<0.01，P<0.05）；能降低去势大鼠子宫细胞的凋亡率（P<0.01，P<0.05），升高子宫Bcl-2 mRNA和蛋白的表达（P<0.01，P<0.05），降低BaxmRNA和蛋白的表达（P<0.01，P<0.05）。[结论]和颜坤泰胶囊有一定的子宫营养作用，可通过调控Bcl-2和Bax基因与蛋白的表达来抑制子宫细胞的凋亡。

Abstract:

[Objective] To investigate the effects of Heyan Kuntai capsules on uterus morphology and apoptosis in ovariectomized rats.[Methods] Female SD rats underwent ovariectomize surgery and were divided into model group, progynova group, Remifemin group and Heyan Kuntai capsule of high, medium and low dose groups (8, 4 and 2 g crude drug/kg respectively). The drugs were administered for 8 weeks. This article observed the impacts of drugs on uterus pathology, staining and apoptosis, detected the expression levels of Bcl-2 and Bax mRNAs and proteins.[Results] It was obtained significantly decreased epithelial thickness and reduced myometrium and endometrium cross-sectional area and wall thickness in the model group compared with the control and Heyan Kuntai capsule treated groups(P<0.01 or P<0.05). It was also decreased Bcl-2 and increased Bax mRNAs and proteins expressions(P<0.01 or P<0.05) in the model group. Further more, the model group showed increased apoptosis rate(P<0.01 or P<0.05) and reduced number of glands(P<0.01 or P<0.05) in the uterus.[Conclusion] Heyan Kuntai capsules elicits uterotrophic effect and attenuates apoptosis in the uterus cells by regulating mRNAs and protein expressions of Bcl-2 and Bax.