基于Ussing Chamber技术的红景天苷大鼠肠黏膜透过性研究

Study on permeabilities of Salidroside via rat mesentery mucosa by using Ussing Chamber

关键词:  红景天苷  Ussing Chamber技术  P-糖蛋白  紧密连接  维拉帕米  十二烷基硫酸钠

Key words:  salidroside  Ussing Chamber  P-gp  tight junction  verapamil  SDS

摘要:

[目的]研究红景天苷在大鼠离体肠黏膜中的透过情况。[方法]采用Ussing Chamber技术，考察不同浓度红景天苷于大鼠肠黏膜透过情况，并考察维拉帕米（Ver）和十二烷基硫酸钠（SDS）对红景天苷大鼠肠黏膜透过的影响。[结果]不同浓度红景天苷在大鼠不同肠段的单位时间转运速率（Flux）随浓度升高而增大，不同肠段表观渗透系数（P_app）无统计学差异（P>0.05）。Ver加入一定量的SDS后，红景天苷在回肠和结肠的P_app显著增加（P<0.01或P<0.05），当SDS浓度为2 g/L时，回肠P_app增加为对照组的2.76倍。[结论]红景天苷在大鼠全肠段均有吸收，且吸收具有浓度依赖性，以被动吸收为主，Ver对红景天苷大鼠各肠吸收无影响，SDS促进了红景天苷回肠和结肠吸收。

Abstract:

[Objective] To investigate the permeability of salidroside in mesentery mucosa of rat ex vivo.[Methods] The Ussing Chamber was employed to investigate the ex vivo permeability in rat mesentery mucosa of salidroside with different concentrations or with the addition of verapamil and SDS.[Results] It was found that the Flux of salidroside was increased with the increasing concentration, and there was no difference(P<0.05) in the apparent permeability coefficient (P_app) in different mesentery segments of rat. The P_app of salidroside got significantly increased in ileum and colon (P<0.01 or P<0.05), and reached to 2.76-fold compared with the control in ileum, when a certain amount of SDS was added in the donator side.[Conclusion] Salidroside could be slightly absorbed in all mesentery segments of rat with a passive concentration-dependent transportation. There were no effects of verapamil on permeation of salidroside, and SDS could significantly increase salidroside permeation in ileum and colon of rat.