不同鼠龄昆明种小鼠流感病毒肺炎动物模型的比较研究

Comparative study on establishment of animal model of influenza virus pneumonia in Kunming mice of different ages

关键词:  小鼠  动物模型  流感病毒

Key words:  mouse  animal model  influenza virus

摘要:

[目的] 通过幼龄鼠与成龄鼠建立流感病毒感染动物模型对照实验研究，探讨幼龄鼠与成龄鼠对流感病毒的易感性。[方法] 采用鼻腔接种甲型流感病毒小鼠，将实验小鼠分为幼龄正常组、幼龄模型组、成龄正常组和成龄模型组4组，观察各组小鼠在一般状态、存活只数、肺组织病变程度等方面的差异，计算肺指数，应用逆转录-聚合酶链反应（RT-PCR）技术检测各组小鼠肺组织的病毒载量。[结果] 1）接种流感病毒后，成龄模型组基本保持较好状态，幼龄模型组一般状态最差，各正常组小鼠状态最好。2）肺组织外观病变程度：各正常组小鼠在肺组织外观全肺野无病变；幼龄模型组肺组织病变最重，与幼龄正常组及成龄模型组比较均有统计学差异（P<0.05）；成龄模型组与成龄正常组比较无统计学差异（P>0.05），与幼龄模型组有统计学差异（P<0.05）.3）肺指数：幼龄模型组肺指数最高，与幼龄正常组及成龄模型组比较均有统计学差异（P<0.05）.4）肺组织IV-RNA的半定量：各正常组小鼠未检测到病毒核酸，幼龄模型组在各时点均检测到大量的病毒核酸，幼龄模型组各时间点与成龄模型组比较有统计学差异（P<0.05）.[结论] 幼龄鼠可以成功塑造小鼠IV感染模型，成龄鼠不能成功造模。

Abstract:

[Objective] Through establishing young and mature mice model with mouse influenza virus (IV) infection to discuss the situation of young mice and mature mice against influenza virus susceptibility. [Methods] Influenza A virus A/FM1/47 was used to infect mice. The mice were divided into young mice and mature mice two groups. General status, survival, the differences of lung lesions, calculated lung index were observed. RT-PCR method was applied to detect IV-RNA viral load differences at two different time points in lung tissues of mice, which was to provide experimental verification for lung often inadequate theory mechanism. [Results] 1) Inoculated with influenza virus, the mature model group basically maintained normal conditions, young model group had worse general states and normal mice had better ones. 2) The appearance of lung lesions showed, the lung tissue of normal mice showed no pathological changes in the appearance of the whole lung field. Compared with the normal group and mature model group, young model group showed the worst changes and had significant differences (P<0.05). There was no significant differences between the mature model group and normal group(P>0.05), but has a significant difference compared with young model group (P<0.05). 3). The lung index showed, young model group were the highest and the comparisons with the normal group and the mature model group were significantly different (P<0.05). 4) The semi-quantitative determination results of IV-RNA in lung tissues showed, there was no virus nucleic acid of IV detected in normal group at each time point. A large number of viral nucleic acid was detected in young model group at each time point. There was a significant difference between young model group and mature model groups at each time point(P<0.05). [Conclusion] IV infection model can shaped successfully in young mice as mature mice unable.