HPLC法同时测定升麻葛根汤中3个有效成分的含量

Simultaneous determination of three active components in decoction of rhizoma cimicifugae and radix puerariae by HPLC

关键词:  升麻葛根汤  高效液相色谱法  异阿魏酸  葛根素  芍药苷

Key words:  decoction of rhizome cimicifugae and radix  HPLC  isoferulic acid  puerarin  paeoniflorin

摘要:

[目的]用高效液相色谱（HPLC）法，建立一种能同时测定升麻葛根汤汤剂中异阿魏酸、葛根素和芍药苷含量的分析方法。[方法]Waters XBridge® Shield RP18色谱柱（250 mm×4.6 mm，5 μm），流动相：甲醇-0.05 mol/L磷酸二氢钾为流动相，梯度洗脱，体积流量为0.8 mL/min，双波长检测，检测波长分别为230 nm、317nm，柱温30℃。[结果]芍药苷、葛根素和异阿魏酸在该色谱条件下能分离良好，且阴性无干扰。其中芍药苷、葛根素和异阿魏酸的线性范围分别为0.382~6.118 μg、1.602~25.626 μg和0.081~1.303 μg；平均回收率（n=6）分别为101.5%、102.9%和98.1%，RSD均小于3.0%。[结论]本方法操作简便、准确、重复性好，可同时测定升麻葛根汤中芍药苷、葛根素和异阿魏酸的含量，为升麻葛根汤的质量评价提供科学依据。

Abstract:

[Objective] To develop an analytical method for simultaneously determining the contents of paeoniflorin, puerarin and isoferulic acid in decoction of rhizoma cimicifugae and radix puerariae by HPLC.[Methods] The separation of three active components were performed on a Waters XBridge column (250 mm×4.6 mm, 5 μm) by using an gradient mobile phase composed of CH₃OH-0.05 mol/L KH₂PO₄, at acolumn temperature of 30℃, the flow rate was 0.8 mL/min, the detection wavelength was 230 nm and 317 nm.[Results] The peaks of paeoniflorin, puerarin and isoferulic acidcompletely resolved very well. The linear ranges of paeoniflorin, puerarin and isoferulic acid were 0.382~6.118 μg, 1.602~25.626 μg and 0.081~1.303 μg, the average recoveries(n=6) were 101.5%, 102.9% and 98.1% respectively, and the RSD was less than 3.0%.[Conclusion] This method is simple, accurate and repeatable. It can be used to simultaneously determine the content of the above three active compounds, as well as to provide a scientific basis for the quality evaluation of the decoction.