癌热宁栓剂直肠给药相关解热机制探索

Exploring the antipyretic mechanism of Airening suppository by rectal drug administration

关键词:  癌热宁栓  癌性发热  作用机制  内生致热源  NF-κB

Key words:  Airening suppository  cancerous fever  mechanism  endogenous pyrogen  NF-κB

摘要:

[目的] 观察癌热宁栓剂的解热机制。[方法] 将家兔随机分为空白对照组、模型对照组、癌热宁栓组和消炎痛栓组4组，采用2，4-二硝基酚致家兔发热模型，测定给药后不同时间的体温变化，采用酶联免疫反应（ELISA）吸附法，分别检测各组家兔血清中白介素-6（IL-6）、环氧化酶（COX-2）、前列腺素E₂（PGE₂）、精氨酸加压素（AVP）、α-黑色素细胞刺激素（α-MSH）的含量，采用免疫组化法半定量观察NF-κB表达的变化。[结果] 消炎痛栓组与癌热宁栓组分别给药后，0.5 h两组较模型对照组体温开始下降（P<0.05），至1 h体温降低明显（P<0.01），COX-2、PGE₂含量显着下降（P<0.05），AVP、α-MSH含量明显升高（P<0.05），肝脏NF-κB的表达下调显着（P<0.05）；癌热宁栓组家兔血清IL-6、COX-2、PGE₂含量显着下降（P<0.05），AVP含量升高明显（P<0.05），肝脏NF-κB的表达明显下调（P<0.05）.[结论] 癌热宁栓剂可显着降低发热家兔的体温，疗效与消炎痛栓相当。癌热宁栓退热作用机制与调控NF-κB通路有关，提示癌热宁栓剂具有多靶点退热作用。

Abstract:

[Objective] To observe the antipyretic mechanism of Airening suppository. [Methods] Rabbits were randomly divided into blank control group, model control group, Airening suppository group Xiaoyantong suppository group. The models of fever induced by 2,4-binitro-phenol, and measured temperature at different times after the treatment. The IL-6, COX-2, PGE₂, AVP, α-MSH were detected in serum of each rabbit by ELISA, and Immunohistochemistry is used to detect the expression of NF-κB of the liver. [Results] After 0.5 h, compared with model control group, the body temperature of experimental groups started to descend (P<0.05), and body temperature descended significantly after 1 h (P<0.01). The contents of COX-2 and PGE₂ were decreased significantly (P<0.05), and the contents of AVP and α-MSH were increased significantly (P<0.05). The contents of COX-2 and PGE₂ were decreased significantly (P<0.05). The expression of liver NF-κB decreased significantly (P<0.05). The contents of IL-6, COX-2 and PGE₂ were decreased significantly of Airening suppository group(P<0.05), and the contents of AVP was increased obviously (P<0.05). The expression of liver NF-κB significantly lowered (P<0.05). [Conclusion] Airening suppository can significantly reduce the body temperature of rabbits, and the curative effect is equivalent to Xiaoyantong suppository. The antipyretic mechanism of Airening suppository may be related to the regulation of NF-κB pathway. It suggests that Airening suppository has a antipyretic function with multiple targets.