| 本文已被：浏览 1427次 下载 1477次 |
码上扫一扫！ |
|
|
| 天芪益智颗粒对阿尔茨海默病大鼠学习记忆能力和突触功能的影响 |
|
刘一川1, 马涛2, 王乐3, 余青4, 孟繁兴5, 张允岭6
|
|
1.中国航空工业集团公司北京航空制造工程研究所门诊部中医科, 北京 100024;2.北京中医药大学东方医院实验中心, 北京 100078;3.北京中医药大学东方医院科研处, 北京 100078;4.北京市丰台区铁营医院皮肤科, 北京 100078;5.北京中医药大学东方医院脑病一科, 北京 100078;6.北京中医药大学东方医院, 北京 100078
|
|
| 摘要: |
| [目的] 研究天芪益智颗粒对阿尔茨海默病大鼠学习记忆能力及脑内突触素、微管相关蛋白-2（MAP-2）表达的影响。[方法] 采用侧脑室注射Aβ1-42的方法建立动物模型，将90只SD大鼠随机分成模型组、假手术组、阳性药组（石杉碱甲）、天芪益智颗粒高、中、低剂量组，每组15只。连续给药30 d后，通过避暗实验评价大鼠的学习记忆能力，采用Western Blot方法测定大鼠脑内突触素、MAP-2.[结果] 与模型组相比，天芪益智颗各剂量组大鼠潜伏期长于模型组（P<0.01），错误次数小于模型组（P<0.01）.模型组大鼠脑内突触素、MAP-2表达低于假手术组（P<0.01），天芪益智颗粒各剂量组突触素、MAP-2表达高于模型组（P<0.01）.[结论] 天芪益智颗粒能改善阿尔茨海默病大鼠学习记忆能力，改善突触功能。 |
| 关键词: 阿尔茨海默病 天芪益智颗粒 突触功能 突触素 微管相关蛋白-2 |
| DOI：10.11656/j.issn.1672-1519.2017.07.13 |
| 分类号:R749.1 |
| 基金项目:国家科技重大专项-重大新药创制项目（2013ZX09103002-002） |
|
| Effects of Tianqi Yizhi granules on learning and memory ability and synaptic function in Alzheimer's disease rats |
|
LIU Yi-chuan1, MA Tao2, WANG Le3, YU Qing4, MENG Fan-xing5, ZHANG Yun-ling6
|
|
1.Department of Traditional Chinese Medicine, Out Patient Department of AVIC Beijing Aeronautical Manufacturing Technology Research Institute, Beijing 100024, China;2.Experiment Center, Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China;3.Division of Scientific Research Management, Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China;4.Department of Dermatology, Tieying Hospital of Fengtai District Beijing, Beijing 100078, China;5.Department of Neurology, Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China;6.Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China
|
| Abstract: |
| [Objective] To investigate the effect of Tianqi Yizhi granules on learning and memory ability of the expression of synaptophysin and MAP-2 in Alzheimer's disease rats. [Methods] Alzheimer's disease animal models were built by injecting Aβ1-42 into the lateral ventricle. The 90 SD rats were randomly divided into 6 groups of 15 rats each, which were model group, sham operation group, medicine group (huperzine), low-dose Tianqi Yizhi granules group, medium-dose Tianqi Yizhi granules group, and high-dose Tianqi Yizhi granules group. After continuously administered for 30 days, step through test were used to evaluate the learning and memory skills of the AD rats. Meanwhile, the expression of synaptophysin and MAP-2 in rats' brain were tested by Western Blot. [Results] In the step through test, the latency was increased in the Tianqi Yizhi granules groups compared with the model group (P<0.01), while the error times decreased (P<0.01). In the model group, the expression of synaptophysin and MAP-2 were both down-regulated (P<0.01), and they were both up-regulated in the Tianqi Yizhi granules groups (P<0.01). [Conclusion] Tianqiyizhi granules had improved the ability of learning and memory of Alzheimer's disease rats and it had up-regulated the expressions of synaptophysin and MAP-2 in AD rats' brain, which indicated that Tianqiyizhi granules had a positive influence on the synaptic function in AD rats. |
| Key words: Alzheimer's disease Tianqi Yizhi granule synaptic function synaptophysin MAP-2 |
