| 摘要: |
| [目的]改进蚓激酶的提取工艺。[方法]从新鲜蚯蚓中经提取、分离和纯化3个工序制备蚓激酶。以酶的活性为指标，依序对比4种溶剂提取方法，4种沉淀分离方法和2种层析纯化方法，得出较优工艺，并运用分级沉淀除杂和凝胶层析除盐进行工艺改进。[结果]优选出缓冲液提取，乙醇分级沉淀分离，DEAE-Sepharose FF和Sephadex G-75层析纯化的蚓激酶提取新工艺，制得蚓激酶酶活28 000 μ/mg，高于旧工艺。[结论]改进后的工艺制备的酶活高，操作简单，适合于工业化生产。 |
| 关键词: 蚓激酶 提取 工艺改进 |
| DOI：10.11656/j.issn.1672-1519.2018.01.17 |
| 分类号:R284.2 |
| 基金项目:江苏省高等学校自然科学研究面上项目（17KJD350003）；泰州市科技支撑（社会发展）项目（SSF20170110）。 |
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| Research on improvement of lumbrokinas extracting process |
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YU Qingbo, SU Pengliang, LIN Ying, LU Dongying, WANG Sainan, MA Yufan, XU Dan
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Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China
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| Abstract: |
| [Objective] To improved extraction process of lumbrokinas.[Methods] Lumbrokinas was preparation from fresh earthworm by extraction, separation and purification. Sequential comparison of four solvent extraction methods, four precipitation separation methods and two chromatography purification methods, to obtaine a better process by investigating enzyme activity. Then the process was improved by fractional precipitation, removal of impurities and gel chromatography.[Results] Lumbrokinas was preparation by optimization of the extraction process, including buffer extraction, ethanol fractionation precipitation, DEAE-Sepharose FF and Sephadex G-75 chromatography. The enzyme activity was 28 000 μ/mg, which was higher than that of the old process.[Conclusion] The improved process has high enzyme activity and simple operation, and is suitable for industrial production. |
| Key words: lumbrokinas extraction process improvement |
