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| UPLC/ESI-Q-TOF MS法分析远志中的化学成分 |
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柴士伟1, 杨帆2,3, 于卉娟2,3, 王跃飞2,3
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1.天津中医药大学第一附属医院, 天津 300193;2.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;3.天津国际生物医药联合研究院, 中药新药研发中心, 天津 300457
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| 摘要: |
| [目的]阐明远志中的化学成分。[方法]采用超高效液相色谱-电喷雾离子化-四级杆飞行时间串联质谱（UPLC/ESI-Q-TOF MS）法分析远志中的化学成分，采用Masslynx V 4.1软件，通过分析准分子离子和二级碎片离子信息，并进一步根据标准品的色谱保留时间和质谱裂解规律，鉴定远志中的化学成分。[结果]鉴定了远志中29个化学成分，主要为口山酮、低聚糖酯、皂苷类成分。[结论]总结归纳了远志药材中主要化学成分的质谱裂解规律，阐明了远志中的主要化学成分。 |
| 关键词: 超高效液相色谱-电喷雾离子化-四级杆飞行时间串联质谱 远志 化学成分 |
| DOI：10.11656/j.issn.1672-1519.2018.01.16 |
| 分类号:R917 |
| 基金项目:天津中医药大学第一附属医院2016年度新医疗新技术项目（2016012）。 |
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| Analysis of chemical constituents of Radix Polygalae by UPLC/ESI-Q-TOF MS |
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CHAI Shiwei1, YANG Fan2,3, YU Huijuan2,3, WANG Yuefei2,3
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1.The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin State Laboratory of Modern Chinese Medicine-Province and Ministry co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;3.Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China
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| Abstract: |
| [Objective] To elucidate the chemical constituents of Radix Polygalae (RP).[Methods] Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF) controlled by Masslynx V 4.1 software was employed for identification of the components in RP. By integrating the information of quasi-molecular and fragment ion, and the retention time and fragment pattern of standards, compounds of RP were identified.[Results] The 29 compounds including xanthones, oligosaccharide esters and saponins of RP were identified.[Conclusion] In this study, the fragment pattern of major compounds in RP was summarized, which provided a basis for further elucidation of chemical substances in RP. |
| Key words: UPLC/ESI-Q-TOF MS Radix Polygalae chemical constituent |
