一贯煎对子痫前期大鼠胎盘NF-κB mRNA与MMP-9 mRNA表达影响

Effect of Yiguanjian on expression of NF-κB mRNA and MMP-9 mRNA in placenta of preeclampsia model rat

关键词:  一贯煎  子痫前期  NF-κB mRNA  MMP-9 mRNA

Key words:  Yiguanjian  preeclampsia  NF-κB mRNA  MMP-9 mRNA

摘要:

[目的] 探讨中药一贯煎对子痫前期大鼠胎盘核转录因子-κB mRNA（NF-κB mRNA）和基质金属蛋白酶-9 mRNA（MMP-9 mRNA）表达的影响。[方法] 建立子痫前期Wistar大鼠模型，并灌胃给予中药一贯煎，监测给药前后大鼠血压及24 h尿蛋白变化。PCR法检测孕鼠胎盘NF-κB mRNA及MMP-9 mRNA的表达。[结果] 1）NF-κB mRNA：与正常妊娠组相比，模型对照组及一贯煎治疗组均升高，与模型对照组相比，一贯煎治疗组降低，且差异均有显著性（P<0.05）。2）MMP-9 mRNA：与正常妊娠组相比，模型对照组与一贯煎治疗组均降低，与模型对照组相比，一贯煎治疗组有所升高，且差异均有显著性（P<0．05）。[结论] 一贯煎通过降低子痫前期大鼠胎盘NF-κB mRNA的表达，升高MMP-9 mRNA的表达治疗子痫前期大鼠。

Abstract:

[Objective] To explore of the effect of Yiguanjian on expression of NF-κB mRNA and MMP-9 mRNA in placenta of preeclampsia model rat.[Methods] The preeclampsia rat model was established and intragastric administration of traditional Chinese medicine Yiguanjian. The changes of blood pressure and 24 h urine protein of rats were monitored before and after dosing. The PCR method to measure the expression of NF-κB mRNA and MMP-9 mRNA in placenta of pregnant rats.[Results] 1) The expression of NF-κB mRNA in model control group and Yiguanjian treat group was higher compared with the normal group, with statistical significance (P<0.05), and the expression in Yiguanjian treat group was lower compared with model contonl group, with statistical significance (P<0.05). 2) The expression of MMP-9 mRNA in model control group and Yiguanjian treat group was lower compared with the normal group, with statistical significance (P<0.05), and the expression in Yiguanjian treat group was higher compared with model control group, with statistical significance (P<0.05).[Conclusion] Yiguanjian treatments preeclampsia model rat by reducing the expression of NF-k-B mRNA and rising the expression of MMP-9 mRNA in placenta.