芪术两活汤治疗痛风性关节炎大鼠实验研究

Experimental study of rat models with gouty arthritis treated by Qizhu Lianghuo decoction

关键词:  芪术两活汤  痛风性关节炎  抗炎作用

Key words:  Qizhu Lianghuo decoction  gouty arthritis  anti-inflammatory effect

摘要:

[目的] 探讨芪术两活汤水提液乙酸乙酯部位、乙醇部位对痛风性关节炎大鼠的作用。[方法] 将90只SD大鼠随机分为9组：空白组、模型组、阿司匹林组、芪术两活汤水提液乙酸乙酯部位低、中、高剂量组及芪术两活汤水提液乙醇部位低、中、高剂量组。造模5 h后开始灌胃给药，观察各组大鼠关节炎症情况，连续给药7 d后取材，测定大鼠血清中尿酸（UA）的含量及血清白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）浓度，HE染色观察各组大鼠关节病理状态，并对单位面积炎症细胞计数。[结果] 芪术两活汤水提液乙醇部位低、中、高剂量组与阿司匹林组在足趾炎症程度显著低于模型组（P<0.01）；乙醇部位低、中、高剂量组及阿司匹林组大鼠血清UA水平及IL-1β、TNF-α浓度显著低于模型组（P<0.01，P<0.05）；与模型组比较，芪术两活汤水提液乙醇部位低、中、高剂量组及阿司匹林组大鼠关节组织病理形态学状态均有显著改善（P<0.05），芪术两活汤水提液乙酸乙酯部位各剂量组与模型组比较，差异均无显著性（P>0.05）；芪术两活汤水提液乙醇部位低、中、高剂量组及阿司匹林组大鼠关节组织炎症细胞计数显著低于模型组（P<0.05），乙酸乙酯部位组与模型组比较差异无显著性（P>0.05）。[结论] 芪术两活汤水提液乙醇部位对痛风性关节大鼠模型具有抗炎作用。

Abstract:

[Objective] To explore the anti-inflammatory of the ethyl acetate fraction and the alcohol fraction of Qizhu Lianghuo decoction (QZLHD) extract on rat models with gouty arthritis.[Methods] Ninety male SD rats were randomly divided into nine groups (ten rats in each group):blank group, model group, aspirin group, ethyl acetate fraction of extracts of QZLHD groups (low-dose group, middle-dose group and high-dose group), alcohol fraction of extracts of QZLHD groups (low-dose group, middle-dose group, high-dose group). After 5 h of modeling, the inflammation of the joints of rats was observed. After 7 d of treatment, the concentrations of UA, IL-1β and TNF-α in serum were determined, the pathological morphology of the joint tissue were observed, and the numbers of inflammation cell per unit area were counted.[Results] The foot inflammation condition of alcohol fraction of QZLHD groups rats and aspirin group rats were significantly lower than those of model group (P<0.01). The concentrations of UA, IL-1β and TNF-α from serum of alcohol fraction of QZLHD groups rats and aspirin group rats were significantly lower than those of model group (P<0.01,P<0.05). Compared with the model group, the pathological morphology of the joint tissue of rats of alcohol fraction of QZLHD groups and Aspirin group both have improved significantly(P<0.05), while those of the ethyl acetate fraction groups have no significant difference (P>0.05). The inflammatory cells among joint tissue in the alcohol fraction groups were significantly lower than that of model group (P<0.05) and there were no significantly difference between the ethyl acetate fraction groups and the model group (P>0.05).[Conclusion] The alcohol fraction of extracts of QZLHD has anti-inflammatory effects on rat models with gouty arthritis.