鱼腥草挥发油对小鼠脑缺血再灌注损伤的保护作用及机制研究

Protective effect and mechanism of volatile oil from Yuxingcao on cerebral ischemia-reperfusion injury in mice

关键词:  鱼腥草挥发油  脑缺血再灌注损伤  机制研究

Key words:  volatile oil of Yuxingcao  cerebral ischemia reperfusion injury  mechanism research

摘要:

[目的] 观察鱼腥草挥发油对小鼠脑缺血再灌注损伤的保护作用及探讨其作用机制。[方法] SPF级C57BL/6小鼠120只，雌雄各半，随机分为假手术组、模型组、辛伐他汀阳性对照组，鱼腥草挥发油高、中、低剂量组，每组20只。造模前连续灌胃给药14 d，其中辛伐他汀阳性对照组灌胃给药20 mg/（kg·d），鱼腥草挥发油低、中、高剂量组分别灌胃给药5、10、20 mg/（kg·d），假手术组、模型组灌胃给予等量生理盐水。末次给药1 h后，除假手术组外，其它各组构建小鼠脑缺血再灌注损伤（MCAO）模型，观察各组小鼠行为学、脑梗死面积、脑水含量和脑海马齿状回的变化，检测血清肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）、总超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量。[结果] 与模型组比较，鱼腥草挥发油低、中、高剂量组明显改善行为学评分和脑海马齿状回神经元的形态学变化，降低脑梗死率、脑水含量和血清TNF-α、IL-8含量（P<0.05），升高血清SOD活性（P<0.05），降低MDA含量（P<0.05），且呈剂量依赖性。辛伐他汀阳性对照组也明显改善上述指标。[结论] 鱼腥草挥发油对小鼠脑缺血再灌注损伤具有保护作用且呈剂量依赖性，其作用机制可能与减轻炎症和抗过氧化损伤有关。

Abstract:

[Objective] To explore the protective effect and mechanism of volatile oil from Yuxingcao on cerebral ischemia reperfusion injury in mice. [Methods] one hundred and twenty SPF C57BL/6 mice were randomly divided into six groups, including Sham-operated group (20), model group (20), simvastatin group (XFTT, 20), low-dose volatile oil of Yuxingcao group (YXC-L, 20), middle-dose volatile oil of Yuxingcao group (YXC-M, 20) and high-dose volatile oil of Yuxingcao group (YXC-H, 20). Fourteen days lavage was done after prophylactic administration. The dose of XFTT group, YXC-L group, YXC-M group and YXC-H were 20 mg/(kg·d), 5 mg/(kg·d), 10 mg/(kg·d) and 20 mg/(kg·d) separately, which given by lavage administration. Sham-operated group and model group gave the same dose of sodium chloride. 1h after last administration, MCAO model was builded in those groups except for Sham-operated group. After the experiment, the change of behavioral evaluation, cerebral infarction area, cerebral water content and hippocampal dentate gyrus were observed, the activity of superoxide dismutase (SOD) and the content of tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8), malondialdehyde (MDA) in serum were measured. [Results] Compared with model group, behavior score and morphological change of neurons in hippocampal dentate gyrus were obviously improved in YXC-L group, YXC-M group and YXC-H group. and decreased cerebral infarction rate, cerebral water content, the content of TNF-α, IL-8 and MDA(P<0.05), while the activity of SOD were increased(P<0.05). which appeared dose-dependent. The above index were improved in XFTT group. [Conclusion] Volatile oil from Yuxingcao has protecting effects on MCAO model mice, and appear dose-dependent.