通腑泻肺方对ALI/ARDS大鼠AQP1/a-ENaC表达的影响

Effect of Tongfu Xiefei recipe on the expression of AQP1/a-ENaC in ALI/ARDS rats

关键词:  通腑泻肺方  急性肺损伤  急性呼吸窘迫综合征  水通道蛋白  上皮细胞钠通道

Key words:  Tongfu Xiefei recipe  acute lung injury  acute respiratory distress syndrome  AQP1  a-ENaC

摘要:

[目的]探讨通腑泻肺方对ALI/ARDS大鼠肺水肿的可能作用机制。[方法]27只大鼠按体质量随机分为空白组、模型组、中药组，每组9只。模型组与中药组尾静脉注射内毒素8 mg/kg，空白组大鼠静脉注射生理盐水。中药组在造模完成后给予通腑泻肺中药灌胃，每次0.01 mL/g，每日1次，共7 d，空白组与模型组予等量生理盐水灌胃。通过病理切片观察各组大鼠肺组织损伤情况，免疫组化染色方法检测肺组织水通道蛋白1（AQP1）和上皮细胞钠通道（a-ENaC）表达。[结果]模型组大鼠肺组织中AQP1和a-ENaC表达水平显著下降（P<0.01）；与模型组相比，中药组AQP1和a-ENaC表达显著升高（P<0.05）。[结论]通腑泻肺方能增强ALI/ARDS大鼠AQP1和a-ENaC表达而促进其肺部水液代谢，从而发挥其改善肺水肿的作用。

Abstract:

[Objective] To investigate the possible mechanism of Tongfu Xiefei Decoction on pulmonary edema in ALI/ARDS rats.[Methods] The 27 rats were randomly divided into blank group, model group and traditional Chinese medicine group. In the model group and the traditional Chinese medicine group, endotoxin was injected intravenously at 8 mg/kg, and the rats in the blank group were injected with normal saline. Chinese medicine group after the completion of modeling to give Tongfu laxative Chinese medicine gavage, each 0.01 mL/g, 1 times/d, a total of 7 days, the blank group and the model group to the same amount of normal saline. The expression of AQP1 and a-ENaC in lung tissue were detected by immunohistochemical staining.[Results] The expression of AQP1 and a-ENaC protein in lung tissue of model group decreased significantly (P<0.01). Compared with model group, the expression of AQP1 and a-ENaC protein in TCM group was significantly higher than that in model group (P<0.05).[Conclusion] Tongluo diarrhea can enhance ALI/ARDS rats AQP1 and a-ENaC protein expression and regulation of its water metabolism, so as to play its role in improving pulmonary edema.