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| 穿心莲内酯通过下调PPARγ-C/EBPα抑制脂滴形成 |
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栗茜1,2, 彭莎1,2, 候宁1,2, 刘敏1,2, 李晶1,2, 张宇昕1,2, 张乔1,2, 王石峰1,2, 张燕玲1,2, 乔延江1,2
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1.北京中医药大学中药学院, 北京 100102;2.国家中医药管理局中药信息工程重点研究室, 北京 100102
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| 摘要: |
| [目的]探讨穿心莲内酯抑制脂肪细胞分化和脂滴堆积作用及机制。[方法]将不同浓度穿心莲内酯分别作用于3T3-L1脂肪细胞，采用高内涵细胞成像技术，分析穿心莲内酯对脂滴形成的作用，并通过检测过氧化物酶体增殖物激活受体γ（PPARγ）和CCAAT/增强子结合蛋白α（C/EBPα）表达分析穿心莲内酯的分子作用机制。[结果]穿心莲内酯剂量依赖降低脂滴堆积，在30 μmol/L可显著降低PPARγ和C/EBPα表达（P<0.01），无显著的细胞抑制作用（P>0.05）。[结论]穿心莲内酯通过降低PPARγ-C/EBPα表达抑制脂肪细胞分化，降低脂滴过度堆积，具有潜在的减轻肥胖作用。 |
| 关键词: 穿心莲内酯 3T3-L1脂肪细胞 PPARγ C/EBPα 脂代谢 |
| DOI：10.11656/j.issn.1672-1519.2018.06.15 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金重点项目（81430094）。 |
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| Andrographolide inhibits lipid droplet accumulation by blocking PPARγ-C/EBPα expression |
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LI Xi1,2, PENG Sha1,2, HOU Ning1,2, LIU Min1,2, LI Jing1,2, ZHANG Yuxin1,2, ZHANG Qiao1,2, WANG Shifeng1,2, ZHANG Yanling1,2, QIAO Yanjiang1,2
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1.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;2.State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China
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| Abstract: |
| [Objective] To investigate the inhibitory effect of andrographolide on adipocyte differentiationand lipid droplet accumulation.[Methods] Different concentrations of andrographolide were added to 3T3-L1 adipocytes. The effect of andrographolide on the formation of fat droplets was performed by highcontent imaging. The molecular mechanism of andrographolide was illustrated by detecting the expression of PPARγ and C/EBPα.[Results] Andrographolide reduced lipid accumulation in dose-dependent manner. The expression of PPARγ and C/EBPα were significantly reduced at 30 μmol/L (P<0.01) without obvious cytotoxicity (P >0.05).[Conclusion] Andrographolide inhibited adipocyte differentiation through blocking PPARγ-C/EBPα expression and reduced excessive lipid droplets accumulation, which holds potential for alleviating obesity. |
| Key words: andrographolide 3T3-L1 adipocyte PPARγ C/EBPα lipid metabolism |
