大蒜素对慢性肾功能衰竭大鼠炎症反应及肾脏组织纤维化的影响

Effects of Allicin on inflammatory response and fibrosis in rats with chronic renal failure

关键词:  大蒜素  慢性肾功能衰竭  炎症  纤维化  抑制作用

Key words:  Allicin  chronic renal failure  inflammatory response  fibrosis  inhibition

摘要:

[目的]观察大蒜素对慢性肾功能衰竭（CRF）大鼠炎症反应和肾脏组织纤维化的影响，探讨大蒜素对CRF大鼠的保护作用机制。[方法]采用腺嘌呤溶液250 mg/（kg·d）灌胃21 d的方法建立CRF动物模型，设模型组，大蒜素5、10、20 mg/（kg·d）组，每组20只，另设正常对照组20只，疗程28 d。测定血清肾功能指标，测定肾脏质量并计算肾脏指数，苏木精-伊红（HE）染色观察肾脏组织病理变化并进行病变评分。酶联免疫吸附（ELISA）法测定血清炎症因子含量水平，测定血清中及肾脏组织中纤维化指标。[结果]大蒜素10、20 mg/（kg·d）组能够显著降低CRF大鼠血清尿素氮（BUN）、肌酐（SCr）、尿酸（UA）含量和尿蛋白（Pro），降低肾脏质量及肾脏指数，明显改善肾脏组织病变、降低肾脏组织病变评分；降低CRF大鼠血清炎症因子C反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α）含量且20 mg/（kg·d）组降低白介素-6（IL-6）含量，降低血浆层黏连蛋白（LN）、PC-Ⅲ、C-Ⅳ并提高血浆纤维连接蛋白（FN），下调I型胶原（Col I）、纤溶酶原激活物抑制物1（PAI-1）表达并上调基质金属蛋白酶-1（MMP-1）；上述差异均具有统计学意义（P<0.05或P<0.01）。[结论]大蒜素可能通过抑制炎症反应和肾脏组织纤维化而对CRF大鼠起到一定的保护作用。

Abstract:

[Objective] To observe the effect of Allicin on the inflammatory response and renal tissue fibrosis in chronic renal failure (CRF) and to study the protective mechanism of Allicin on CRF rats.[Methods] The CRF rat models were established by intragastric administration of adenine solution[250 mg/(kg·d)] for 21 days, setting model group and allicin[5, 10, 20 mg/(kg·d)] group, n=20; and setting normal control group(n=20). Monitoring indicators of serum renal function were determined, the kidney index was calculated, the pathological changes of renal tissue was observed by HE staining and the Lesion score was determined. The content of inflammatory factors in serum and the content of fibrosis index in surem and in renel tissue were determined.[Results] Compared with model group, the content of BUN, SCr, UA, Pro in serum of allicin 10, 20 mg/(kg·d) groups were significantly reduced; renal index were significantly reduced, Significant improvement of renal tissue lesions, lower renal tissue lesions score; decreased the content of CRP, IL-6, TNF-α in serum, decreased the LN, PC-Ⅲ, C-IV and increased the FN; down-regulating the expression of Col I, PAI-1 and up-regulating the expression of MMP-1; all of the difference above were significant (P<0.05 or P<0.01).[Cnclusion] llicin may play a protective role on CRF rats by inhibiting the renal tissue fibrosis by inhibiting inflammatory response and renal fibrosis.