扶肾方对尿毒症大鼠腹膜间充质TGF-β1、E-cadherin和α-SMA表达的影响

Effects of Fushen recipe on expression of TGF-β1, E-cadherin and α-SMA in peritoneal mesenchymal membranes of uremia rats

关键词:  扶肾方  腹膜纤维化  腹膜透析

Key words:  Fushen recipe  peritoneal fibrosis  peritoneal dialysis

摘要:

[目的]探讨扶肾方对尿毒症大鼠腹膜间充质转化生长因子-β1（TGF-β1）、E-钙黏素（E-cadherin）和α-平滑肌肌动蛋白（α-SMA）表达的影响。[方法]采用SD大鼠切除5/6肾脏+1.5%百特腹透液的方法复制大鼠腹膜纤维化模型。扶肾方干预6周后，检测血清学指标肌酐和尿素氮的含量；苏木精-伊红（HE）染色观察腹膜病理改变；逆转录-聚合酶链反应（RT-PCR）法检测各组腹膜间皮标志物E-cadherin和α-SMA的表达；酶联免疫吸附（ELISA）法检测TGF-β1含量的变化。[结果]扶肾方干预后，各组血清肌酐及尿素氮均呈现下降趋势，尤其以高剂量组最为明显（P<0.05）；扶肾方组腹膜间皮细胞变性、脱落，炎症细胞浸润以及血管新生现象明显减少；RT-PCR检测结果显示，模型组E-cadherin较正常组表达下降，差异有统计学意义（P<0.05），TGF-β1、α-SMA较正常组表达上调，差异有统计学意义（P<0.01），与模型组相比，扶肾方干预后的各组E-cadherin表达均有所上调，且以高剂量组上调最为明显（P<0.01）；TGF-β1、α-SMA中、高剂量组有统计学差异（P<0.05或P<0.01），其他组较正常组有下调趋势，低剂量组结果无统计学意义，中、高剂量组结果有统计学差异（P<0.05）。[结论]扶肾方很可能通过抑制TGF-β1，调节E-cadherin和α-SMA表达，从而抑制腹膜间皮细胞间质转化的发生。

Abstract:

[Objective] To invigorate the kidney in uremic rats TGF-β1, expression of E-cadherin and α-SMA.[Methods] SD rats underwent 5/6 +1.5% renal dialysis Baxter replication in rat model of peritoneal fibrosis. After 6 weeks' intervention, the serum levels of creatinine and urea nitrogen were detected. The pathological changes of peritoneum were observed by HE staining. The expression of E-cadherin and α-SMA in peritoneal mesothelial markers in each group was detected by RT-PCR method, and the content of TGF-β1 was detected by ELISA.[Results] The results help kidney prescription intervention, serum creatinine and urea nitrogen were decreased, especially in the high dose group was most significant (P<0.05); Fushen recipe Group of peritoneal mesothelial cell degeneration, shedding, inflammatory cell infiltration and neovascularization were obviously reduced; the results of RT-PCR showed that E-cadherin decreased in model group compared with normal group the expression, the difference was statistically significant (P<0.05); TGF-β1 and α-SMA compared with normal group expression, the difference was statistically significant (P<0.01), compared with the model group, the intervention group to help kidney. The expression of E-cadherin was increased, and the high dose group increased most significantly (P<0.01); TGF-β1 and α-SMA in middle and high dose groups had statistically significant difference (P<0.05, P<0.01); the other group compared with control group, low dose group decreased, the results were not statistically significant and medium and high dose group had statistically significance (P<0.05).[Conclusion] Fushen recipe may inhibit the occurrence of PMCs EMT by inhibiting TGF-β1 and regulating the expression of E-cadherin and α-SMA.