三七总皂苷对杠柳毒苷药代动力学的影响

Effects of Panax notoginsenosides on pharmacokinetics of Periplocin

关键词:  杠柳毒苷  三七总皂苷  LC-MS/MS  药代动力学

Key words:  Periplocin  Panax notoginsenosides  LC-MS/MS  pharmacokinetics

摘要:

[目的]建立液相色谱-质谱联用（LC-MS/MS）定量分析方法，考察三七总皂苷对杠柳毒苷在大鼠体内的药动学影响。[方法]将18只大鼠随机分成杠柳毒苷单独给药组、杠柳毒苷和低剂量三七总皂苷配伍组、杠柳毒苷和高剂量三七总皂苷配伍组，大鼠连续口服给药7 d，在最后1次给药前和给药后不同的时间点从眼底静脉丛取血，采用LC-MS/MS法，以补骨脂素为内标，测定大鼠体内杠柳毒苷的含量，并运用DAS 1.0软件计算待测物的药动学参数。[结果]杠柳毒苷在1~500 ng/mL的范围内线性关系良好，方法学符合要求。药动学结果表明，杠柳毒苷配伍三七总皂苷后，杠柳毒苷在大鼠血浆中的主要药动学参数C_max、AUC_0-t、AUC_0-∞与杠柳毒苷单独给药相比存在统计学差异。[结论]本研究所建立的LC-MS/MS方法快速、灵敏、准确，可用于杠柳毒苷在大鼠体内的药动学研究。三七总皂苷对杠柳毒苷在大鼠体内的药动学存在影响。

Abstract:

[Objective] To establish a LC-MS/MS method for determination of Periplocin and study the effect of Panax notoginsenosides on pharmacokinetics of Periplocin in rats.[Methods] Eighteen rats were divided into Periplocin alone group; Periplocin combined with low-dose and high-dose Panax notoginsenosides groups. All rats were orally administered for seven consecutive days, blood samples were obtained from orbitalis at different time before and after the last time administration. LC-MS/MS method was developed and validated for the determination of Periplocin in rat plasma using psoralen as the internal standard, and main pharmacokinetic parameters of analyte were processed by DAS1.0 software.[Results] Calibration curves of Periplocin were linear over 1~500 ng/mL, the method validation was in line with requirements. Results of pharmacokinetics showed that there were significant differences between the pharmacokinetic parameters of Periplocin in rats with or without Panax notoginsenosides.[Conclusion] The LC-MS/MS method was rapid, sensitive and accurate, and it was successfully applied to evaluate the pharmacokinetics of Periplocin in rats. And the Panax notoginsenosides had effect on pharmacokinetic of Periplocin.