| 摘要: |
| [目的]研究益胃汤对初老雌性大鼠下丘脑产生调节作用的相关蛋白质，在分子水平上探索益胃汤的作用机制。[方法]将20只4～6月龄雌性大鼠随机分成正常组、去势组，每组10只，10只10～12月龄雌性大鼠作为初老组。给药结束后，取3组大鼠下丘脑组织，运用同位素标记相对和绝对定量技术（iTRAQ）分离、分析、鉴定大鼠下丘脑差异蛋白。[结果]分析比较初老组、去势组、正常组3组大鼠下丘脑蛋白质，通过多组数据韦恩图分析发现初老组与去势组有17种共同表达的差异蛋白。通过火山图（Volcano Plot）过滤，确定显著差异蛋白[差异倍数（FC）>1.5或<2/3，P<0.05]；筛选出了7种与衰老相关的显著差异蛋白及其相关KEGG通路，它们分别为：plasminogen、α-crystallin B chain、Hsp70 binding protein 1、serum transferrin、unconventional myosin-Id、myelin oligodendrocyte glycoprotein、protein Fbxo44。[结论]益胃汤对这7种显著差异蛋白起到上调或下调的作用，为研究益胃汤延缓衰老作用的靶蛋白奠定了基础。 |
| 关键词: 益胃汤 延缓衰老 差异蛋白 iTRAQ 下丘脑 |
| DOI：10.11656/j.issn.1672-1519.2018.10.14 |
| 分类号:R285.5 |
| 基金项目:山东省中医药科技发展计划项目（2015-395）。 |
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| Differential protein analysis of Yiwei decoction on hypothalamus in precocious female rats |
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ZHANG Yingjie1, WANG Peng2, ZHANG Meiying3, SUI Kaiyue1, LU Fang1
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1.Zibo Hospital of Integrated Traditional Chinese and Western Medicine, Zibo 255000, China;2.Shandong University of Traditional Chinese Medicine, Jinan 250014, China;3.Tianjin University of Traditional Chinese Medicine Affiliated Wuqing Chinese Medicine Hospital, Tianjin 301700, China
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| Abstract: |
| [Objective] To study the associated protein of Yiwei decoction on the regulation of hypothalamus in precocious female rats and to explore the mechanism of Yiwei decoction at the molecular level.[Methods] Twenty female rats aged 4~6 months were randomly divided into two groups:normal group and castration group. Ten female rats aged 10~12 months were used as the old group. After the administration, the hypothalamus tissue of three groups of rats was taken and iTRAQ was used to isolate, analyze and identify the differential proteins of rat hypothalamus using isotopic labeling relative and absolute quantitative techniques.[Results] The hypothalamus proteins in the rats of the three groups were compared and analyzed. According to the Venn diagram analysis of several groups of data, there were 17 differentially expressed proteins expressed in the precocious group and the castration group. Through Volcano Plot filtration, significant differences in protein (FC>1.5 or <2/3, P<0.05) were determined; seven types of senescence-related proteins and their related KEGG pathways were screened out. They were plasminogen, α-crystallin B chain, Hsp70 binding protein 1, serum transferrin, unconventional myosin-Id, myelin oligodendrocyte glycoprotein, and protein Fbxo44.[Conclusion] Yiwei decoction plays an important role in up-regulating or down-regulating these seven kinds of significantly different proteins, which lays a foundation for studying the effect of Yiwei decoction in delaying the aging of target proteins. |
| Key words: Yiwei decoction anti-aging differential proteins iTRAQ hypothalamus |
