| 摘要: |
| [目的]研究当归补血汤对糖尿病大鼠肝脏、骨骼肌中蛋白激酶C-ζ（PKC-ζ）表达的影响。[方法]选取雄性SD大鼠50只，随机分为空白对照组、当归补血汤对照组、2型糖尿病（T2DM）模型组、T2DM+当归补血汤组、2型糖尿病+吡格列酮组。采用链脲佐菌素（STZ）联合高脂高糖饲料建立大鼠糖尿病模型，造模成功后，药物干预5周，取血检测大鼠空腹血糖（FPG）、空腹胰岛素（FINS）水平。用免疫组织化学法检测各组大鼠肝脏、骨骼肌细胞中PKC-ζ表达。[结果]与空白对照组比，T2DM模型组FPG、FINS显著升高；与T2DM模型组相比，T2DM+当归补血汤组和T2DM+吡格列酮组FPG、FINS下降。通过免疫组化检测发现，与空白对照组比，T2DM模型组肝脏和骨骼肌中PKC-ζ的表达下降；T2DM+当归补血汤组和T2DM+吡格列酮组较T2DM组表达升高，且T2DM+当归补血汤组与T2DM+吡格列酮组组间无明显差异。[结论]当归补血汤能够改善胰岛素抵抗、降低血糖，这可能与其促进肝脏、骨骼肌中PKC-ζ的表达有关。 |
| 关键词: 当归补血汤 糖尿病 胰岛素抵抗 蛋白激酶C-ζ |
| DOI：10.11656/j.issn.1672-1519.2018.11.15 |
| 分类号:R587.1 |
| 基金项目:湖北省自然科学基金项目（2012FFB04323）；湖北省卫生厅中西医结合科研项目（2010Z-Z04）。 |
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| Effect of Danggui Buxue decoction on the expression of PKC-ζ in the liver and skeletal muscle of diabetic rats |
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AI Wang, ZHOU Zhen, ZHANG Manling, WANG Xiuping, ZHANG Yingwen
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Department of Traditional Chinese Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
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| Abstract: |
| [Objective] To explore the effect of Danggui Buxue decoction on the protein kinase C-ζ expression in the liver and skeletal muscle cell of diabetic rats.[Methods] Fifty male SD rats were randomly divided into normal control group (NC group), Danggui Buxue decoction control group (DG group), type 2 diabetes mellitus group (T2DM group), type 2 diabetes mellitus + Danggui Buxue Decoction group (T2DM + DG group), type 2 diabetes + pioglitazone group (T2DM + BG group). Type 2 diabetes mellitus rat models were established by high fat diets and intraperitoneal injection with sreptozotocin (STZ). After 5 weeks, measuring fasting blood glucose (FPG) and fasting insulin (FINS) in blood and using immunohistochemical method to detect the content of PKC-ζ in the liver, skeletal muscle in rats seach group.[Results] Compared with NC group, the FPG and FINS of T2DM group increased significantly. Compared with T2DM group, the FPG and FINS of T2DM+DG group and T2DM+BG group decreased. The expression of PKC-ζ in the liver and skeletal muscle cell membrane of T2DM group was decreased by immunohistochemistry, while the expression of PKC-ζ in T2DM+DG group and T2DM + BG group was higher than that of T2DM group. Except that, there was no significant difference between T2DM+DG group and T2DM +BG group.[Conclusion] Danggui Buxue decoction can improve insulin resistance and reduce blood sugar, which may be related to promoting the expression of PKC-ζin the liver and skeletal muscle cells. |
| Key words: Danggui Buxue decoction diabetes insulin resistance PKC-ζ |
