心脑舒通胶囊对氧糖剥夺/复氧星形胶质细胞神经营养因子分泌的影响及对神经元的保护作用

Effect of Xinnao Shutong Capsules on secretion of neurotrophic factor in oxygen-glucose deprivation/reoxyge nation astrocytes and protection of neurons

关键词:  心脑舒通胶囊  星形胶质细胞  神经元  脑源性神经营养因子  胶质源性神经营养因子

Key words:  Xinnao Shutong Capsules  astrocyte  neuron  brain derived neurotrophic factor  glial cell-derived neurotrophic factor

摘要:

[目的]探讨心脑舒通胶囊对氧糖剥夺/复氧（OGD/R）处理的星形胶质细胞（C8-D1A）神经生长因子分泌的影响，以及研究心脑舒通药物处理的OGD/R胶质细胞条件培养液对神经元细胞（Neuro-2A）的保护作用。[方法]CCK-8检测心脑舒通对正常培养和OGD/R胶质细胞活力的影响；酶联免疫吸附（ELISA）法检测心脑舒通对OGD/R胶质细胞的脑源性神经营养因子（BDNF）、胶质源性神经营养因子（GDNF）、睫状神经营养因子（CNTF）释放的影响；CCK-8检测心脑舒通处理的胶质细胞条件培养液对OGD/R神经元细胞活力的影响。[结果]1）心脑舒通对正常培养的胶质细胞活力无显著影响，但可以提高OGD/R后胶质细胞的增殖活力。2）0.1 μg/mL心脑舒通可以显著提高OGD/R胶质细胞BDNF的释放。3）0.1 μg/mL心脑舒通药物处理的胶质细胞条件培养液可提高OGD/R神经元的存活能力。[结论]心脑舒通对OGD/R损伤后的胶质细胞有一定保护作用，其处理后的胶质细胞条件培养液对神经元有一定的保护作用。

Abstract:

[Objective] To explore the effect of Xinnao Shutong Capsules on the secretion of nerve growth factor in astrocytes cell induced by oxygen-glucose deprivation/reoxygenation (OGD/R). The protective effects on OGD/R-induced neurons cell variability treated by astrocytes conditioned medium were also explored.[Methods] The effect of Xinnao Shutong Capsules on both normal culture and OGD/R-induced C8-D1A cell viability were detected by CCK-8. ELISA was adopted to measure the secretion of BDNF, GDNF and CNTF in OGD/R-induced C8-D1A cell which treatment with Xinnao Shutong Capsules.[Results] 1) Xinnao Shutong Capsules had no significant effect on the viability of normal cultured C8-D1A cell, but could increase the proliferation of OGD/R-induced C8-D1A cell. 2) Xinnao Shutong Capsules (0.1 μg/mL) could significantly promote the secretion of BDNF on C8-D1A cell after OGD/R. 3) C8-D1A cell treated with Xinnao Shutong Capsules (0.1 μg/mL) conditioned medium had protective effect on neurons.[Conclusion] Xinnao Shutong Capsules had protective effect on OGD/R-induced C8-D1A cell, and its treated C8-D1A conditioned medium played protective role on OGD/R-induced neurons.