| 摘要: |
| [目的]探讨槲皮黄酮对原代心肌细胞肥大、线粒体功能及动力学的影响。[方法]原代培养大鼠心肌细胞，采用血管紧张素Ⅱ（AngⅡ）及槲皮黄酮共同干预细胞48、72 h后，BCA试剂盒检测细胞中蛋白浓度，倒置显微镜分析心肌细胞直径；酶标仪检测心肌细胞内三磷酸腺苷（ATP）及活性氧（ROS）含量，JC-1方法分析心肌细胞线粒体膜电位（MMP）；蛋白免疫印迹（Western Blot）方法测定线粒体动力学相关蛋白视神经萎缩蛋白（OPA1）、动力相关蛋白（Drp1）及磷酸化动力相关蛋白1（p-Drp1）表达量。[结果]与空白组比较，模型组心肌细胞中总蛋白浓度、细胞直径大小及ROS水平显著升高（P<0.05），ATP和MMP值则显著降低（P<0.05），Western Blot结果发现OPA1蛋白表达水平显著降低（P<0.05），p-Drp1蛋白表达水平显著升高（P<0.05）；与模型组比较，槲皮黄酮处理72 h后心肌细胞中总蛋白浓度、细胞直径大小及ROS水平显著降低（P<0.05），ATP和MMP值则显著升高（P<0.05），Western Blot结果发现OPA1蛋白表达水平显著增高（P<0.05），p-Drp1蛋白表达水平显著降低（P<0.05）。[结论]槲皮黄酮对AngⅡ引起的心肌细胞肥大具有保护作用，其作用机制可能与降低心肌细胞线粒体功能障碍、稳定线粒体动力学平衡相关。 |
| 关键词: 槲皮黄酮 心肌细胞 肥大 线粒体 |
| DOI：10.11656/j.issn.1672-1519.2019.01.22 |
| 分类号:R285.5 |
| 基金项目: |
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| Effects of quercetin on mitochondrial function and dynamics in process of improving myocardial cell hypertrophy |
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WU Shun1, CHEN Mingjun1, ZHOU Yan2
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1.Department of Endocrinology, People's Hospital of Xinzhou District, Wuhan 430400, China;2.Department of Cardiology, People's Hospital of Xinzhou District, Wuhan 430400, China
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| Abstract: |
| [Objective] To observe the effects of quercetin on the cell hypertrophy, mitochondrial function and dynamics in cardiocytes.[Methods] Primary myocardial cells were cultured with angiotensin Ⅱ (Ang Ⅱ) and quercetin for 48 or 72 h. The total protein content was detected using BCA kit, and cell diameter was measured by inverted microscope. The contents of adenosine triphosphate (ATP) and reactive oxygen species (ROS) in cardiocytes were detected using microplate reader. Mitochondrial membrane potential (MMP) was measured by JC-1. Mitochondrial optic atrophy (OPA1), dynamin-related protein 1 (DRP1) and phosphorylation dynamin-related 1 (p-Drp1) was determined by Western Blot assay.[Results] Compared with control group, the total protein content, cell diameter and ROS were greatly increased in model group, while the ATP and MMP were significantly decreased (P<0.05). Western Blot results indicated that the expression of OPA1 significantly decreased, while the expression of p-Drp1 greatly increased in model group (P<0.05). Compared with model group, the total protein content, cell diameter and ROS were greatly decreased in cardiocytes after treatment with quercetin for 72 h, while the ATP and MMP were significantly increased (P<0.05). Western Blot results indicated that the expression of OPA1 significantly increased, while the expression of p-Drp1 greatly decreased in in cardiocytes after treatment with quercetin for 72 h (P<0.05).[Conclusion] Quercetin can improve myocardial cell hypertrophy induced by Ang Ⅱ, and the mechanism may related to alleviating myocardial mitochondrial dysfunction and dynamic unbalance. |
| Key words: quercetin cardiocyte hypertrophy mitochondria |
