| 摘要: |
| [目的]观察麦门冬汤含药血清对促进大鼠骨髓间充质干细胞（BMSC）向肺泡上皮细胞分化的影响。[方法]分别予麦门冬汤大鼠血清、肺纤维化模型大鼠血清、肺纤维化模型大鼠+麦门冬汤血清对绿色荧光蛋白标记的骨髓间充质干细胞（GFP-BMSC）进行干预14 d，通过免疫组化、实时荧光定量聚核酶链反应（qPCR）方法，检测各组GFP-BMSC细胞肺表面活性蛋白（SP-C）、水通道蛋白-5（AQP-5）蛋白及mRNA的表达。[结果]模型组、模型+麦门冬汤组SP-C、AQP5蛋白表达明显高于DMEM对照组、麦门冬汤组（P<0.01），其中模型+麦门冬汤组SP-C、AQP5表达明显高于模型组（P<0.01）。[结论]麦门冬汤在肺纤维化环境中可促进GFP-BMSC向肺泡上皮细胞分化，该作用可能是其改善肺纤维化的重要机制。 |
| 关键词: 麦门冬汤 骨髓间充质干细胞 肺纤维化 干细胞分化 |
| DOI：10.11656/j.issn.1672-1519.2019.02.20 |
| 分类号: |
| 基金项目:广西自然科学基金青年基金项目（2013GXNSFBA019144）；国家自然科学基金地区科学基金项目（81860829）。 |
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| Effect of Maimendong Decoction on bone marrow mesenchymal stem cells(BMSC) to differentiate into alveolar epithelial cells of rat |
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LIU Rui1, LEI Ningning2, WU Juanjuan1, YE Chuandong2, CHEN Sining1
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1.Department of Geriatrics, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China;2.Guangxi University of Chinese Medicine, Nanning 530011, China
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| Abstract: |
| [Objective] To observe the effect of Maimendong Decoction on bone marrow mesenchymal stem cells(BMSC) to differentiate into alveolar epithelial cells of rats.[Methods] Maimendong Decoction rat serum, rat model of pulmonary fibrosis in rats serum and rat model of pulmonary fibrosis+Maimendong Decoction serum were given on GFP-BMSC. After 14 days, to detect the expression of SP-C, AQP-5 protein and mRNA in GFP-BMSC cells by immunohistochemistry and qPCR method.[Results] The expression of SP-C and AQP-5 proteins in the model group, model+Maimendong Decoction group was significantly higher than that of DMEM control group, Maimendong Decoction group (P<0.01); while the model+Maimendong Decoction group SP-C, AQP-5 proteins expression was significantly higher than the model group (P<0.01).[Conclusion] Maimendong Decoction can promote GFP-BMSC cells into alveolar epithelial cells in pulmonary fibrosis, which may be one of the important mechanisms to improve pulmonary fibrosis. |
| Key words: Maimendong Decoction bone marrow mesenchymal stem cells pulmonary fibrosi stem cells differentiation |
