内异止痛方对子宫内膜异位症大鼠相关mRNA E-cadherin、mRNA β-catenin的影响

Effect of Neiyi Zhitong Prescription for relieving pain on E-cadherin and β-catenin gene transcription in EMs rat

关键词:  子宫内膜异位症  黏附  β-catenin  E-cadherin  内异止痛方

Key words:  endometriosis  adhesion  β-catenin  E-cadherin  Neiyi Zhitong Prescription

摘要:

[目的]采用逆转录-聚合酶链反应（RT-PCR）技术检测子宫内膜异位症（EMs）模型大鼠异位病灶组织中β-catenin、E-cadherin基因转录水平，探讨内异止痛方对EMs模型大鼠β-catenin、E-cadherin的影响。[方法]取SD大鼠57只，采用自体移植法构建EMs模型，于4周后开腹观察模型构建是否成功，并取模型大鼠5只，利用苏木精-伊红（HE）染色法对其进行组织病理学评价。将40只造模成功的EMs大鼠，随机分为4组，即模型组、中药高、低剂量组、阳性药物组，每组10只，另设假手术组8只（仅进行开腹切除手术，不进行自体移植）。中药高、低剂量治疗组分别灌胃985.3 mg/（kg·d）和245.8 mg/（kg·d）内异止痛方，阳性药物组灌胃7.2 mg/（kg·d）达那唑，模型组及假手术组灌胃2 mL/（kg·d）生理盐水，各组每天均给药1次，连续用药4周；采用RT-PCR技术检测EMs模型大鼠异位病灶组织中β-catenin、E-cadherin转录水平。[结果]与模型组比较，内异止痛方能够显著升高EMs模型大鼠E-cadherinmRNA并降低β-catenin mRNA转录水平，且中药高剂量组各基因转录水平明显优于低剂量组（P<0.01）。[结论]内异止痛方可通过上调E-cadherin或下调β-catenin基因转录来维持E-cad/β-cat复合体结构和功能的稳定性，而达到加强在位内膜细胞间黏附的作用。

Abstract:

[Objective] The expression of β-catenin and E-cadherin gene in ectopic focus tissue of rats with EMs was detected by RT-PCR technique by constructing rat model of endometriosis. To investigate the therapeutic effect of Neiyi Zhitong Prescription on β-catenin and E-cadherin.[Methods] The 57 SD rats were selected and EMs model was constructed by autologous transplantation. The success of the model construction was observed by laparotomy 4 weeks later,and 5 model rats were selected for histopathological evaluation by HE staining. The 40 EMs rats successfully modeled were randomly divided into 4 groups,model group,high-dose and low-dose Chinese medicine treatment group,and positive drug group,with 10 rats in each group and 8 rats in the sham operation group (open resection only,no autologous transplantation). In the high-dose and low-dose groups of traditional Chinese medicine,985.3 mg/(kg·d) and 245.8 mg/(kg·d) by Neiyi Zhitong prescription were given. The positive drug group was given 7.2 mg/(kg·d) danazole by gavage,and the model group and the sham operation group were given 2 mL/(kg·d) normal saline by gavage. Each group was given the drug once a day for 4 weeks. Transcription levels of β-catenin and E-cadherin in ectopic lesion tissues of EMs model rats were detected by RT-PCR.[Results] Compared with the model group,Neiyi Zhitong Prescription can significantly increase the mRNA of E-cadherin and reduce the transcription level of β-catenin mRNA in EMs model rats,and the transcription level of each gene in the high-dose group of traditional Chinese medicine is significantly better than that in the low-dose group (P<0.01).[Conclusion] Neiyi Zhitong Prescription can maintain the structural and functional stability of the E-cad/β-cat complex by up-regulating the transcription of E-cadherin or down-regulating the transcription of β-catenin gene,thus enhancing the adhesion between cells in the endometrium.