滇黄精对急性肺损伤模型大鼠的炎症因子及体内氧自由基的影响

Effect of Polygonatum kingianum on inflammatory factors and oxygen radical of acute lung injury model rat

关键词:  滇黄精  急性肺损伤性大鼠  炎症因子  氧自由基

Key words:  Polygonatum kingianum  acute lung injury rat  inflammatory factors  oxygen radicals

摘要:

[目的]探讨滇黄精对急性肺损伤模型大鼠的炎性因子及体内氧自由基的影响。[方法]50只雄性SD大鼠随机分为5组，包括正常对照组（NCG）、模型组（NMG）和滇黄精低（LLG）、中（MLG）、高剂量（HLG）实验组（5、10、15 mg/kg），每组10只。气管内滴灌脂多糖（LPS，5 mg/kg）方法建立急性肺损伤（ALI）模型大鼠，造模成功4 h后给模型大鼠腹腔注射不同浓度的滇黄精水提液，24 h后采集动脉血，检测大鼠的动脉血氧分压（PaO₂）、肺泡灌洗液中炎性细胞的数量、丙二醛（MDA）和髓过氧化物酶（MPO）浓度以及谷胱甘肽过氧化酶（GSH-Px）和超氧化物歧化酶（SOD）的活性。酶联免疫吸附法（ELISA）检测大鼠肿瘤坏死因子-α（TNF-α）、白介素-10（IL-10）、白介素-1β（IL-1β）、白介素-6（IL-6）的含量。取肺部组织检测肺生理相关指标肺系数（LI）、肺含水率[（W-D）/W]以及左肺湿/干质量比（W/D），以期探讨滇黄精对急性肺损伤模型大鼠的炎性因子和氧自由基影响。[结果]与空白对照相比，模型组大鼠的LI、W/D、（W-D）/W指数低，而注射了滇黄精的实验组比模型组高（P<0.01），PaO₂结果相反，这种趋势有剂量依赖性；模型组大鼠的各种炎性细胞数量比空白对照组高（P<0.01），而各实验组大鼠炎性细胞数量低于模型组（P<0.05），高剂量组数量明显低于中剂量组（P<0.05）。炎性因子检测结果表明，模型鼠的IL-1β、IL-6、TGF-α水平高于空白对照组，IL-10比空白组低（P<0.05），而实验组大鼠的IL-1β、IL-6、TGF-α水平比模型组高（P<0.05），IL-10水平低于模型组，高剂量组与中剂量组相比，差异有统计学意义（P<0.01）。模型组大鼠肺泡灌洗液中MDA和MPO水平明显高于空白对照组，SOD和GSH-Px活性低（P<0.05），实验组大鼠MDA、MPO水平低于模型组，SOD、GSH-Px活性明显高于模型组（P<0.05），高剂量组和中剂量组相比，差异具有统计学意义（P<0.05）。[结论]中药滇黄精可以有效缓解LPS诱导急性肺损伤性大鼠的肺部损伤症状，改善炎症反应水平，减轻ALI大鼠肺组织的氧自由基损伤，而且这种效应有剂量依赖性。

Abstract:

[Objective] To investigate the effect of Polygonatum kingianum on inflammatory factors and oxygen radical of acute lung injury model rats.[Methods] The 50 SD male rats were randomly divided into 5 groups, normal control group (NCG), normal model group(NMG), low level group(LLG), middle level group(MLG) and high level group(HLG). Acute lung injury (ALI) model rats were made by intratracheal drip irrigating LPS (5 mg/kg). The Polygonatum kingianum water extract was injected into rats' enterocoelia 4 hours after modeling. 24 hours later, arterial blood was collected and PaO₂, amounts of inflammatory cells in alveolar lavage fluid, levels of MOD, MPO, GSH-Px and SOD were measured. ELISA was introduced to test the level of TNF-α, IL-10, IL-1β and IL-6. The LI,W/D and (W-D)/W were tested to value the biological function of rats' lung.[Results] Compared with the NCG, NMG group showed lower levels of LI,W/D and (W-D)/W (P<0.01), and the level in experimental group injected Polygonatum kingianum was higher than NMG (P<0.01). The level of PaO₂ was on the contrary, and the trend had dose dependence. The inflammatory cells in NMG group were more than NCG(P<0.01), which in each experimental group were lower than NMG (P<0.05), the number in HLG was higher than MLG obviously (P<0.05). The results of inflammatory factors showed the IL-1β, IL-6 and TGF-α level in NMG was higher than NCG, but IL-10 was lower (P<0.05). The IL-1β, IL-6 and TGF-α level in each experimental group was higher than NMG and IL-10 was lower (P<0.05). The difference was significant between HLG and MLG (P<0.01). MDA and MPO levels in alveolar lavage fluid of the NMG were significantly higher than those of the NCG, and lower activity of SOD and GSH-Ps (P<0.05). The level of MDA, MPO of experimental groups were lower than NMG, activity of SOD and GSH-Px were higher than NMG (P<0.01). The difference was significant between HLG and MLG(P<0.05).[Conclusion] Chinese medicine of Polygonatum kingianum can relief symptoms of thoracic injury induced by LPS, optimizing inflammation levels, reducing oxygen free radical injury of ALI rats' lung tissue, and the dose-dependence is obvious.