| 摘要: |
| [目的]探讨槲皮素通过Wnt/β-catenin信号通路对人宫颈癌上皮细胞增殖及迁移的影响及其可能的作用机制，为抑制宫颈癌进展的临床治疗提供新的靶点。[方法]人宫颈癌上皮细胞系HeLa细胞为研究对象，给予不同浓度槲皮素处理24 h后，通过CCK-8实验检测槲皮素对HeLa细胞增殖能力的影响。采用Transwell及细胞划痕实验检测槲皮素对HeLa细胞垂直及水平运动能力的影响。免疫荧光法检测β-catenin表达及TopFlash荧光素酶报告系统检测槲皮素对HeLa细胞Wnt/β-catenin信号通路的抑制作用。[结果]CCK-8检测表明，槲皮素处理组HeLa细胞增殖率明显低于对照组（P<0.01），且抑制作用具有浓度依赖性。槲皮素处理组HeLa细胞垂直及水平运动能力均较对照细胞降低，差异有统计学意义（P<0.01）。对照组HeLa细胞中β-catenin蛋白密集分布于胞核和胞质中，而槲皮素处理组中β-catenin蛋白主要分布于细胞质中。TopFlash荧光素酶报告系统表明槲皮素处理组HeLa细胞Wnt/β-catenin信号通路受到抑制。[结论]槲皮素可能通过抑制Wnt/β-catenin信号通路活化抑制宫颈癌细胞的增殖和迁移。 |
| 关键词: 槲皮素 宫颈癌细胞 Wnt/β-catenin信号通路 细胞增殖 细胞迁移 |
| DOI：10.11656/j.issn.1672-1519.2019.02.25 |
| 分类号: |
| 基金项目:国家自然科学基金项目（81360145）。 |
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| Experimental research of quercetin inhibits proliferation and migration of human cervical cancer cells via inhibiting β-catenin |
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WANG Xiangqing1, MA Zhenjun1, BAO Hongyun1, WANG Ru1, CHEN Xuelian1, LIU Zhiyong2, KONG Fangfang1
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1.Department of Gynaecology and Obstetrics, 464 Hospital of the PLA, Tianjin 300381, China;2.Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
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| Abstract: |
| [Objective] To investigate the effects of quercetinon proliferation and migration of human cervical cancer (CC) cells by Wnt/β-catenin signaling pathway and its mechanism and to provide a new gene target in the treatment of CC.[Methods] The research objects were the human CC HeLa cells. After treatment with different concentrations ofquercetin for 24 hours, the proliferation of HeLa cells was detected by CCK-8 test, the horizontal and vertical migration were detected by Transwell and wound scraching assays, the activation of Wnt/β-catenin signaling pathway was detected by β-catenin immunofluorescence assay and TOP-flash luciferase reporter system.[Results] CCK-8 test indicated that the cell proliferation rate of the quercetin group was significantly lower than the control group (P<0.01), and the inhibition of quercetinon proliferation was dose-dependent. The horizontal and vertical migration of Hela cells in the quercetin group was significantly decreased compared to the control group(P<0.01). The β-catenin immunofluorescence was distributed in nuclear and cytoplasm in the control cells, while mainly in the cytoplasm in the quercetin-treated cells. Top Flashluciferase reporter system indicated the Wnt/β-catenin signaling pathway was inhibited in the quercetin-treated cells.[Conclusion] Quercetin may inhibit proliferation and migration of human cervical cancer cells via inhibiting Wnt/β-catenin signaling pathway. |
| Key words: quercetin cervical cancer cell Wnt/β-catenin signaling pathway cell proliferation cell migration |
