芹菜素对慢性肾功能衰竭大鼠贫血和电解质代谢紊乱的影响

Effects of apigenin on anemia and disorders of electrolyte metabolism in chronic renal failure rats

关键词:  芹菜素  慢性肾功能衰竭  贫血  电解质  肾功能

Key words:  allicin  chronic renal failure  anemia  electrolytes  kidney function

摘要:

[目的]研究芹菜素（APG）对慢性肾功能衰竭（CRF）大鼠贫血和电解质代谢紊乱的影响。[方法]将100只SD大鼠按照随机数字表法随机分为正常对照组、模型组、APG低剂量组[10 mg/（kg·d）]、APG中剂量组[20 mg/（kg·d）]、APG高剂量组[40 mg/（kg·d）]组，每组20只；采用腺嘌呤连续灌胃21 d的方法复制CRF模型大鼠，APG各剂量组腹腔注射给药治疗（1次/日），正常对照组和模型组同步给予生理盐水，疗程28 d。测定肾功能指标[血尿素氮（BUN）、血肌酐（SCr）、尿素（UA）]，称体质量并计算肾脏指数；测定贫血指标[红细胞（RBC）、血红蛋白（HGB）、红细胞压积（HCT）、血清铁（SI）、总铁结合力（TIBC）、铁蛋白（FER）]；测定血清电解质[钾（K⁺）、钠（Na⁺）、钙（Ca²⁺）、磷（P³⁺）]；通过苏木精-伊红（HE）染色对肾脏组织进行病理学检查。[结果]与模型组比较，经APG中、高剂量治疗28 d后能够有效降低CRF模型大鼠血清BUN、SCr、UA含量；降低肾脏指数；提高HGB、HCT和SI、TIBC、FER，其中APG高剂量组RBC显著升高；提高血清Ca²⁺含量并降低P³⁺含量，其中APG高剂量组K⁺、Na⁺显著降低；上述差异均具有统计学意义（P<0.05或P<0.01）。病理学检查结果显示，经APG治疗能够有效抑制CRF大鼠肾脏组织病变。[结论]APG具有改善CRF大鼠贫血、电解质紊乱的药理学作用，这可能是APG对肾功能衰竭能够起到一定保护作用的机制之一。

Abstract:

[Objective] To study the effect of Apigenin (APG) on anemia and disorders of electrolyte metabolism in chronic renal failure(CRF) rats.[Methods] 100 SD rats were randomly divided into normal control group,model group and APG low-dose[10 mg/(kg·d)],medium-dose[20 mg/(kg·d)],high-dose[40 mg/(kg·d)] groups,n=20;the CRF rat model was established by intragastric administration of adenine solution for 21 days,the drugs were given by intraperitoneal injection for 28 days,once a day,while the normal control group and model group were given saline simultaneously at the same time. The levels of serum urea nitrogen (BUN),serum creatinine (SCr) and uric acid (UA) were measured,the kidney index was calculated. The level of red blood cells (RBC),hemoglobin (HGB),hematocrit (HCT),serum iron (SI),total iron binding capacity (TIBC) and ferritin (FER) in whole blood were measured;the level of potassium (K⁺),sodium (Na⁺),calcium (Ca²⁺) and phosphorus (P³⁺) in serum were measured. The pathological changes of renal tissue was observed by HE staining.[Results] Compared with model group,the contents of BUN,SCr,UA in serum of APG medium-/high-dose groups were significantly reduced;renal index was significantly reduced;the levels of RBC,HGB,HCT,SI,TIBC,FER were significantly increased;the level of Ca²⁺ in serum was significantly increased and the levels of K⁺,Na⁺,P³⁺ were significantly decreased;all of the difference above were significant (P<0.05 or P<0.01). Pathological examination showed that APG treatment can effectively inhibit renal tissue lesions in CRF rats.[Conclusion] APG can improve the pharmacological effects of anemia and electrolyte disturbance in CRF rats,which may be one of the mechanisms that APG can play a protective role against renal failure.