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| 元胡止痛口服液对原发性痛经的镇痛作用及机制研究 |
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张恺1,2, 李娜娜3, 李嵩4, 孟庆芬5, 关建丽5, 邓昊1,2, 樊官伟1,2,6
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1.天津中医药大学第一附属医院, 天津 300193;2.天津市中医方证转化研究重点实验室, 天津 301617;3.天津市中医药研究院附属医院, 天津 300120;4.天津中医药大学研究生院, 天津 301617;5.河南福森药业有限公司, 淅川 474450;6.天津中医药大学中医药研究院, 天津 301617
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| 摘要: |
| [目的]观察元胡止痛口服液对原发性痛经的治疗作用并探讨其作用机制，为其在临床治疗原发性痛经提供实验依据。[方法]采用缩宫素诱导原发性痛经大鼠模型，考察不同剂量元胡止痛口服液对动物模型扭体反应的影响，并检测大鼠血浆雌二醇（E2）、孕酮（Prog）、丙二醛（MDA）、超氧化物歧化酶（SOD）与谷胱甘肽过氧化物酶（GSH-Px）水平，以及子宫组织前列腺素F2α（PGF2α）、前列腺素E2（PGE2）、血栓素B2（TXB2）、6-酮-前列腺素F1α（6-keto-PGF1α）、钙离子（Ca2+）与一氧化氮（NO）含量。[结果]元胡止痛口服液可显著减少痛经大鼠模型的扭体次数（P<0.05），降低扭体发生率。与模型组大鼠相比，元胡止痛口服液治疗组大鼠血浆MDA水平显著降低（P<0.05），子宫组织PGF2α水平与Ca2+含量显著下调（P<0.05），且子宫6-keto-PGF1α水平显著升高（P<0.05）。[结论]元胡止痛口服液对原发性痛经大鼠模型具有显著的镇痛作用，其作用机制可能与调节血浆MDA水平及子宫组织内PGF2α、6-keto-PGF1α、Ca2+含量相关。 |
| 关键词: 元胡止痛口服液 缩宫素 原发性痛经 镇痛作用 |
| DOI：10.11656/j.issn.1672-1519.2019.10.18 |
| 分类号:R271.11 |
| 基金项目:天津市杰出青年科学基金（17JCJQJC46200）。 |
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| Analgesic effect and pharmacological mechanisms of Yuanhu Zhitong Oral Liquid on primary dysmenorrhea |
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ZHANG Kai1,2, LI Nana3, LI Song4, MENG Qingfen5, GUAN Jianli5, DENG Hao1,2, FAN Guanwei1,2,6
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1.First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin Key Laboratory of Translational Research of TCM Prescription and Syndrome, Tianjin 301617, China;3.Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China;4.Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;5.Henan Fusen Pharmaceutical Co., Ltd., Henan 474450, China;6.Institute of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| [Objective] To investigate the effects and pharmacological mechanisms of Yuanhu Zhitong Oral Liquid (YZOL) on primary dysmenorrhea and provide experimental basis for its clinical application.[Methods] The rat model of primary dysmenorrhea was induced by oxytocin. The writhing response was used as an indicator to investigate the effects of different doses of YZOL on dysmenorrhea rats. Plasma levels of estradiol (E2),progesterone (Prog),malondialdehyde (MDA),superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured in rats to explore the mechanisms of YZOL treatment. Moreover,the levels of prostaglandin F2α (PGF2α),prostaglandin E2 (PGE2),thromboxane B2 (TXB2),6-keto-prostaglandin F1α (6-keto-PGF1α),Ca2+ and nitric oxide (NO) in the uterus of rats were examined.[Results] YZOL could significantly reduce the writhing times (P<0.05) and writhing incidence of dysmenorrhea rats. Compared with the model group,significantly lower levels of MDA,PGF2α,Ca2+ (P<0.05) and markedly higher activity of 6-keto-PGF1α (P<0.05) were observed in the YZOL-treated group.[Conclusion] These results indicated that YZOL had a significant analgesic effect on the rat model of primary dysmenorrhea,and its mechanisms might be closely linked to the regulation of plasma MDA level and the levels of PGF2α,6-keto-PGF1α,Ca2+ in the uterine tissue. |
| Key words: Yuanhu Zhitong Oral Liquid oxytocin primary dysmenorrhea analgesic effect |
