芪茵荷叶饮对糖耐量异常大鼠糖代谢及骨骼肌NF-κB信号通路的影响

Effect of Qiyin Heye Decoction on glucose metabolism and NF-κB signaling pathway in skeletal muscle of rats with abnormal glucose tolerance

关键词:  芪茵荷叶饮  糖耐量受损  正交实验  NF-κBp65

Key words:  Qiyin Heye Decoction  impaired glucose tolerance  skeletal muscle  NF-κBp65

摘要:

[目的]探讨补气益脾组方芪茵荷叶饮对糖耐量异常大鼠糖代谢及骨骼肌NF-κB信号通路的影响。[方法]采用高脂高糖饲料建立糖耐量受损（IGT）大鼠模型，将成模大鼠按体质量随机分为模型组（M）和9组不同剂量配伍组（A、B、C、D、E、F、G、H和I）。采用正交实验设计，选取黄芪、茵陈蒿和荷叶进行三因素三水平实验L₉（3⁴）。于干预前及干预6周、12周检测大鼠空腹血糖（FBG）、空腹胰岛素（FINS）及胰岛素抵抗指数（HOMA-IR），观察骨骼肌组织病理变化，实时荧光定量PCR方法检测各组大鼠肌肉组织IκB-α和NF-κBp65基因转录水平。[结果]D组FBG在干预12周时低于给药前水平；与M组比较，各实验组FINS在干预6周、12周（除A和B组）下降明显（P<0.05），HOMA-IR在干预6周时A、D、E、F、G、H组和干预12周时D、F、G、H、I组较M组下降显著（P<0.05）。仅不同剂量的茵陈蒿对FBG影响存在统计学差异，仅不同剂量黄芪对FINS和HOMA-IR调节具有统计学差异，主次顺序为黄芪>茵陈蒿>荷叶。M组大鼠骨骼肌水肿、有炎性细胞浸润，G组NF-κBp65mRNA表达水平较M组明显下降（P<0.05）。[结论]芪茵荷叶饮中黄芪为君、茵陈蒿为臣，辅以荷叶，最佳配伍方案为半量黄芪、2倍量茵陈蒿、半量荷叶。IGT大鼠骨骼肌组织存在炎性反应，G组配伍的芪茵荷叶饮可通过抑制NF-κB信号通路，改善IGT大鼠胰岛素抵抗，预防IGT向2型糖尿病（T2DM）演变。

Abstract:

[Objective] To discuss the effects of Qiyin Heye Decoction on glucose metabolism and NF-κB signaling pathway in skeletal muscle of rats with abnormal glucose tolerance.[Methods] A rat model of impaired glucose tolerance was established by using high-fat and high-sugar diet. The model rats were randomly divided into model control group 1 group and different dose compatibility group 9 groups according to their weight,and a normal control group was set. In this study,orthogonal experiment was used to select three factors of astragalus,capillaris and lotus leaf for three-level experiment L₉(3⁴). Fasting blood glucose,fasting insulin and insulin resistance index were measured before and 6 and 12 weeks after intervention. The transcription levels of IκB-α and NF-κBp65 gene in muscle tissue of each group were detected by real-time fluorescent quantitative PCR,and pathological changes of skeletal muscle tissue were observed.[Results] Compared with the model control group,the insulin resistance index was significantly decreased (P<0.05) in group A,D,E,F,G,and H after 6 weeks of administration and in group D,F,G,H,and I after 12 weeks of administration. The insulin resistance index showed a significant difference with time (P<0.01). Rats in the model control group had skeletal muscle edema and inflammatory cell infiltration,but the expression level of NF-κBp65 was significantly decreased compared with the control group (P<0.05).[Conclusion] There was an inflammatory response in skeletal muscle tissue of IGT rats,but the NF-κB signaling pathway was not activated. Qiyin Heye Decoction can improve insulin resistance in IGT rat model and prevent the evolution of IGT to T2DM,but its mechanism needs further study.