基于RhoA/ROCK-1通路研究针刺干预SHR脑保护作用机制

Study on the brain protective mechanism of acupuncture intervention on SHR based on RhoA/ROCK-1 pathway

关键词:  高血压  脑损害  RhoA  ROCK-1

Key words:  hypertension  brain damage  RhoA  ROCK-1

摘要:

[目的] 明确自发性高血压大鼠（SHR）脑损害与RhoA、ROCK-1的相关性，探讨针刺干预SHR脑保护的Rho通路机制。[方法] 将SHR随机分为模型对照组、太冲穴组，WKY大鼠设为正常对照组。太冲穴组针刺干预4周，其余两组在干预期内给予相同程度、相同时长的抓取。观测各组大鼠收缩压、舒张压、平均动脉压的改变；苏木精-伊红（HE）染色观察脑组织形态学改变；蛋白免疫印迹（Western blot）法测定各组大鼠脑组织中RhoA、ROCK-1的表达水平。[结果] 在干预周期内，正常对照组收缩压、舒张压、平均压均处于正常血压范围内。与正常对照组比较，模型对照组收缩压、舒张压、平均压均较高，且有随着周龄增加而增高的趋势。与模型对照组相比，太冲穴组可有效降低收缩压，有降低舒张压、平均压的趋势。模型对照组较正常对照组脑组织细胞排列紊乱，细胞坏死及间质水肿程度加重，RhoA、ROCK-1表达升高；太冲穴组与模型对照组比较，脑细胞排列规则，细胞坏死及间质水肿消失，RhoA、ROCK-1表达下降。[结论] 针刺太冲穴能有效降低SHR血压，改善脑损害，其机制可能与抑制RhoA/ROCK-1信号通路表达相关。

Abstract:

[Objective] To determine the correlation between SHR brain damage and RhoA and rock-1,and to explore the Rho pathway mechanism of acupuncture intervention in SHR brain protection.[Methods] SHR rats were randomly divided into model control group,taichong acupoint group,and WKY rats were set as blank control group. The acupuncture intervention in the taichong point group lasted for 4 weeks,and the other two groups were given the same degree and the same time quantum of grasping during the intervention period. The changes of systolic blood pressure,diastolic blood pressure and mean arterial pressure were observed. Morphological changes of brain tissue were observed by HE staining. The expression levels of RhoA and rock-1 were determined by Western blot.[Results] During the intervention period,the systolic,diastolic,and mean pressures of the normal control group were within the normal blood pressure range. Compared with the normal control group,the systolic blood pressure,diastolic blood pressure and mean blood pressure of the model control group were higher,and there was a tendency to increase with the increase of the weeks of conception. Compared with the model control group,the Taichong group can effectively reduce systolic blood pressure and has the trend of reducing diastolic blood pressure and average pressure. Compared with the normal control group,the model control group was disordered in cell tissue,the degree of cell necrosis and interstitial edema was aggravated,and the expression of RhoA and ROCK-1 was increased. Compared with the model control group,the taichong group had regular arrangement of brain cells,cell necrosis and interstitial edema disappeared and the expression of RhoA and ROCK-1 decreased.[Conclusion] Acupuncture taichong can effectively reduce SHR blood pressure and improve brain damage,and its mechanism may be related to the inhibition of RhoA/rock-1 signaling pathway expression.