京万红软膏对大鼠糖尿病足烫伤的疗效及机制研究

Effect and mechanism of Jingwanhong Ointment on scald of diabetic foot in rats

关键词:  京万红软膏  糖尿病足  烫伤  PDGF  TGF-β

Key words:  Jingwanhong Ointment  diabetic foot  scald  PDGF  TGF-β

摘要:

[目的] 探讨京万红软膏对大鼠糖尿病足烫伤的作用，并初步考察其作用机制。[方法] 复制糖尿病大鼠模型，继而复制大鼠糖尿病足烫伤模型。实验分为4组：假手术组、模型组、京万红软膏组、重组人表皮生长因子外用溶液（rhEGF）组。给药14 d，记录给药前后大鼠足部宏观形态、体积及瓦格纳（Wagner）分级结果。给药后检测足部组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）的含量及血小板源性生长因子（PDGF）、转化生长因子-β（TGF－β） mRNA的表达情况。[结果] 造模后，模型组的足趾体积、Wagner分级、SOD含量与PDGF mRNA的表达与假手术组相比较，差异具有显著性（P<0.01）。与模型组相比较，给药5 d后，京万红软膏组可显著减小糖尿病大鼠的足趾体积（P<0.05），给药14 d后，京万红软膏组可明显促进溃疡创面组织及表皮的修复，极显著降低Wagner分级水平（P<0.01），显著升高足部组织中SOD的含量及上调PDGF mRNA的表达（P<0.05）。[结论] 京万红软膏针对大鼠糖尿病足烫伤具有控制感染、祛除坏死组织、促进组织修复等多重功效，其愈合作用可能与消除过量的氧自由基及上调PDGF mRNA的表达相关。

Abstract:

[Objective] To investigate the effect of Jingwanhong Ointment on scald of diabetic foot in rats and its mechanism.[Methods] The diabetic rat model was copied,and then diabetic foot scald model was established. The experiment was divided into four groups:sham operation group,model group,Jingwanhong Ointment group,recombinant human epidermal growth factor external solution (rhEGF) group. After 14 days of administration,the macromorphology,volume and Wagner grading results of rats' feet were recorded before and after administration. The content of superoxide dismutase (SOD),malondialdehyde (MDA),nitric oxide (NO) and the expression of platelet-derived growth factor (PDGF) and transforming growth factor-β (TGF-β) mRNA in foot tissue were measured after administration.[Results] After modeling,the toe volume,Wagner classification,SOD content,and PDGF mRNA expression in the model group were significantly different from those in the sham operation group (P<0.01). Compared with the model group,Jingwanhong Ointment group could significantly reduce the toe volume of diabetic rats (P<0.05) after 5 days of administration. After 14 days of administration,Jingwanhong Ointment group could significantly promote the repair of ulcer wound tissue and epidermis,significantly reduce Wagner classification level (P<0.01),significantly increase the content of SOD in foot tissue and up-regulate the expression of PDGF mRNA (P<0.05).[Conclusion] Jingwanhong Ointment has multiple effects on controlling diabetic foot scald in rats,controlling infection,removing necrotic tissue,and promoting tissue repair. Its healing effect may be related to eliminating excess oxygen free radicals and up-regulating PDGF mRNA expression.