中成药抑制巨噬细胞炎性细胞因子释放的体外研究

Study of the effect of marketed Chinese patent medicine on inhibiting release of inflammatory factors in macrophage models

关键词:  中成药  RAW264.7细胞  脂多糖  细胞因子炎症

Key words:  Chinese patent medicine  RAW264.7 cell  LPS  cytokine inflammation

摘要:

[目的] 病毒感染后免疫系统过度激化导致的"细胞因子风暴"是病毒性肺炎由轻症向重症转换的重要原因，同时也是重症患者产生并发症的重要原因。该实验旨在寻求能有效抑制细胞因子的中成药，为病毒性肺炎治疗提供科学依据。[方法] 采用脂多糖（LPS）诱导RAW264.7细胞建立体外炎症损伤模型，用Griess法检测细胞上清液中一氧化氮（NO）生成量，酶联免疫法检测细胞因子肿瘤坏死因子-α（TNF-α）、白介素（IL）-1β、IL-2、IL-6、IL-10分泌量，对收集的已上市中成药开展抑制炎症因子释放的研究。[结果] 发现牛黄清心丸（局方）、藿香正气软胶囊、牛黄解毒丸、穿心莲内酯滴丸等中成药对LPS诱导RAW264.7细胞一氧化氮（NO）生成及细胞因子分泌均有显著抑制作用，提示这4种中成药具有抑制炎症因子活性。[结论] 中成药牛黄清心丸（局方）、藿香正气软胶囊、牛黄解毒丸、穿心莲内酯滴丸具有抑制"细胞因子风暴"作用，在防治重症肺炎中具有潜在的临床应用价值。

Abstract:

[Objective] The "cytokine storm" caused by immune system hyper-activation after viral infection is an important reason for the conversion of viral pneumonia from mild to severe,and also the important reason for complications in severe patients. In this paper,we screen marketed Chinese patent medicines with significant inhibition in cytokines secretion are provide scientific basis for the treatment of viral pneumonia.[Methods] We investigated the inhibition of inflammatory factors of marketed medicines in lipopolysaccharide (LPS)-induced RAW264.7 cells model. To detect the production of nitric oxide (NO) by Griess method,detect secretion of cytokines TNF-α,IL-1β,IL-2,IL-6,IL-10 by the enzymatic immunoassay kits.[Results] The results here report that Niuhuang Qingxin pill,Huoxiang Zhengqi soft capsule,Niuhuang Jiedu pill,and Andrographolide dropping pill can inhibit NO production and cytokine secretion in LPS induced RAW264.7 cells,suggesting that these marketed Chinese patent medicine have the activity of inhibiting inflammatory factors.[Conclusion] Niuhuang Qingxin pill,Huoxiang Zhengqi soft capsule,Niuhuang Jiedu pill,and Andrographolide dropping pill have the effect of inhibiting "cytokine storm",and have more promising clinical applications.