优化新生脉散方治疗心力衰竭的网络药理学机制分析

Analysis of network pharmacology mechanism of Optimized New Shengmai Powder in treating heart failure

关键词:  优化新生脉散方  心力衰竭  网络药理学  机制分析

Key words:  Optimized New Shengmai Powder  heart failure  network pharmacology  mechanism analysis

摘要:

[目的] 运用网络药理学方法分析优化新生脉散方治疗慢性心力衰竭（CHF）的作用机制。[方法] 基于中药系统药理数据库和分析平台（TCMSP）、BATMAN-TCM及UniProt数据库查询优化新生脉散方药物的主要活性成分与相应靶点，利用GeneCards数据库检索CHF相关靶点，通过OmicShare网站分析并结合Cytoscape-v3.6.0软件构建优化新生脉散方中药-化合物-靶点-疾病网络图，使用DAVID数据库对潜在靶点进行基因功能（GO）及京都基因和基因组百科全书（KEGG）通路富集分析。[结果] 根据类药性（DL）、口服吸收利用度（OB）、参数评分（Scorecutoff）筛选出与CHF相关的潜在化合物85种及靶点89个，其中，化合物有quercetin、luteolin、kaempferol等，靶点包括SCN5A、ADRB2、NOS2等。功能富集分析涉及生物过程、分子功能、细胞组分的GO条目共497个，主要参与调控细胞凋亡、增殖及缺氧、炎症反应等方面，KEGG通路富集得到139条，主要与缺氧诱导因子-1（HIF-1）、磷脂酰肌醇3激酶/蛋白激酶B（PI3K-Akt）、肿瘤坏死因子（TNF）信号通路等有关。[结论] 优化新生脉散方治疗CHF具有多成分、多靶点、多途径的作用，为进一步研究优化新生脉散方治疗CHF的潜在复杂机制提供参考方向。

Abstract:

[Objective] To analyze the mechanism of Optimized New Shengmai Powder in treating of chronic heart failure (CHF) by network pharmacology.[Methods] Based on Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP),BATMAN-TCM and UniProt database,the main active components and corresponding targets of Optimized New Shengmai Powder were acquired,using GeneCards database to retrieve CHF related targets,through the analysis of OmicShare website and combining with the software of Cytoscape-v3.6.0,the network diagram of traditional Chinese medicine-potential compounds-potential targets-disease of Optimized New Shengmai Powder was constructed,the Gene function (GO) and Kyoto gene and genome encyclopedia (KEGG) pathways of potential targets were enriched using the DAVID database.[Results] According to Drug-likeness (DL),Oral bioavailability (OB) and Score cutoff,85 potential compounds and 89 targets related to CHF were screened,among them,there were quercetin,luteolin,kaempferol,and the targets included SCN5A,ADRB2,NOS2,etc. Functional enrichment analysis included a total of 497 GO items involving biological processes,molecular functions and cellular components,which were mainly involved in the regulation of apoptosis,proliferation,hypoxia and inflammatory response. 139 KEGG pathways were enriched,which are mainly related to the HIF-1,PI3K-Akt and TNF signaling pathways.[Conclusion] The Optimized New Shengmai Powder for CHF treatment has the function of multiple components,multiple targets and multiple pathways,which provides a reference direction for further study on the potential complex mechanism of Optimized New Shengmai Powder for CHF treatment.