仙藕乳蒲方对糖尿病大鼠皮肤溃疡创面愈合及线粒体的影响

Effect of Xian'ou Rupu Recipe on skin ulcer wound healing and mitochondria in diabetic rats

关键词:  仙藕乳蒲方  糖尿病溃疡  线粒体  ATP  ROS

Key words:  Xian'ou Rupu Recipe  diabetic ulcer  mitochondria  ATP  ROS

摘要:

[目的] 探讨仙藕乳蒲方对糖尿病大鼠皮肤溃疡创面愈合及线粒体的影响。[方法] 将48只雄性SD大鼠通过灌胃高脂乳剂、注射链尿佐菌素（STZ）、制作背部全层皮肤溃疡制成糖尿病大鼠溃疡模型，随机分为模型对照组、仙藕乳蒲方组，仙藕乳蒲方组敷以仙藕乳蒲方粉末，模型对照组不予处理。造模后第3、7、14天大鼠观察后处死取材，透射电镜观察创面组织成纤维细胞线粒体超微结构，酶联免疫吸附测定（ELISA）各组创面三磷酸腺苷（ATP）、活性氧分子（ROS）含量并进行统计学分析。。[结果] 第7、14天仙藕乳蒲方组创面愈合率均高于模型对照组（P<0.05）。超微结构示仙藕乳蒲方组第3天线粒体肿胀程度较模型对照组轻，少数嵴消失。第7天线粒体肿胀轻度减轻，较多可见清楚嵴结构的线粒体。第14天线粒体数量丰富且结构清楚；而模型对照组第7天线粒体结构破坏更严重，伴聚集、变形、融合等改变。第14天肿胀线粒体数量减少，部分可见清楚嵴结构。ELISA结果，第7、14天，仙藕乳蒲方组ATP均高于模型对照组，ROS均低于模型对照组（P<0.05）。。[结论] 仙藕乳蒲方能防护线粒体损伤，促进线粒体生成ATP并抑制ROS过度产生，改善线粒体能量供应，降低创面氧化应激水平，进而促进糖尿病溃疡大鼠创面愈合。

Abstract:

[Objective] To investigate the effect of Xian'ou Rupu Recipe on skin ulcer wound healing and mitochondria in diabetic rats.[Methods] Forty-eight male SD rats were made into a diabetic rat ulcer model by intragastric administration of high-fat emulsion,injection of streptozotocin (STZ),and production of full-thickness skin ulcers on the back. There were randomly divided into the model control group and the Xian'ou Rupu Recipe group. The Xian'ou Rupu Recipe group was coated with the powder of Xian'ou Rupu Recipe,while the model control group were not treated. On the 3rd,7th and 14th day after modeling,the rats were sacrificed and collected. The ultrastructure of mitochondria in wound fibroblasts was observed by transmission electron microscope,and the contents of adenosine triphosphate (ATP) and reactive oxygen species (ROS) in wound were measured by enzyme-linked immunosorbent assay (ELISA) and statistically analyzed.[Results] The wound healing rate of the Xian'ou Rupu Recipe group on the 7th and 14th days was higher than that of the model control group (P<0.05). The ultrastructure showed that the swelling degree of the Xian'ou Rupu Recipe group on the 3rd day was lighter than that of the model control group,and a few ridges disappeared. On the 7th day,the mitochondrial swelling of the Xian'ou Rupu Recipe group was slightly reduced,and the mitochondria of the clear ridge structure were more visible. On the 14th day,mitochondria were abundant and had clear structure. But on the 7th day,mitochondrial structure of model control group was broken more seriously,accompanied by aggregation,deformation,fusion and other changes. On the 14th day,the number of swollen mitochondria decreased,and some of them showed clear ridge structure. The results of ELISA showed that ATP was higher and ROS was lower in Xian'ou Rupu Recipe group than that in model control group on the 7th and 14th day (P<0.05).[Conclusion] Xian'ou Rupu Recipe can protect against mitochondrial damage,promote mitochondrial production of ATP and inhibit excessive production of ROS,improve mitochondrial energy supply,reduce the level of oxidative stress in wounds,and promote wound healing in diabetic ulcer rats.