丹酚乙酸镁对大鼠肢体缺血再灌注损伤炎性因子及其mRNA调控作用的探讨

Study on the regulation effect of magnesium salvianolic acetate on inflammatory factors and mRNA limbs ischemia-reperfusion injury in rats

关键词:  缺血再灌注  炎性因子  丹酚乙酸镁  肢体

Key words:  ischemia-reperfusion  inflammatory factor  magnesium salvianolic acetate  limb

摘要:

[目的] 通过肢体缺血再灌注损伤期血清中促炎细胞因子白细胞介素6（IL-6）、肿瘤坏死因子-α（TNF-α）、白细胞介素1β（IL-1β）、白细胞介素10（IL-10）水平及其mRNA表达的变化规律，探讨丹酚乙酸镁对肢体缺血再灌注损伤保护作用的机制。[方法] 构建大鼠肢体缺血再灌注损伤模型。将大鼠分为4组，比较不同组别中肢体缺血再灌注损伤期炎性细胞因子IL-6、TNF-α、IL-1β和IL-10水平及其mRNA的变化规律。判断丹酚乙酸镁对炎性细胞因子及其mRNA的影响。[结果] 丹酚乙酸镁处理组中标本内TNF-α，IL-6及IL-1β水平及其mRNA表达较对照组明显降低，IL-10水平及mRNA表达明显升高。[结论] 丹酚乙酸镁通过上调抗炎性细胞因子水平及mRNA表达，降低血浆中炎性细胞因子的浓度，使得缺血再灌注过程中组织的损伤程度得以减轻，从而起到保护作用。

Abstract:

[Objective] To investigate the protective effect of magnesium salvianolate acetate on limb ischemia-reperfusion injury,though investigating the changes of serum proinflammatory cytokines interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β),interleukin-10 (IL-10) and their mRNA expression during limb ischemia-reperfusion injury.[Methods] A rat model of limb ischemia-reperfusion injury was established. The rats were divided into four groups,and the levels of inflammatory cytokines IL-6,TNF-α,IL-1β and IL-10 and the changes of mRNA in different groups during limb ischemia-reperfusion injury were compared. Then determined the effect of magnesium salvianolic acetate on inflammatory cytokines and their mRNA.[Results] The levels of TNF-α,IL-6 and IL-1β and their mRNA expression in the magnesium salvianolic acetate treatment group were significantly lower than those in the control group,and the IL-10 level and mRNA expression were significantly increased.[Conclusion] Magnesium salvianolate acetate can decrease the concentration of inflammatory cytokines in plasma by up regulating the level and mRNA expression of anti-inflammatory cytokines,which can reduce the degree of tissue injury during ischemia-reperfusion,thus playing a protective role.