| 摘要: |
| [目的] 本研究探讨姜黄素抑制淋巴管生成相关因子的可能分子机制。[方法] MTT检测MGC803细胞增殖情况，实时定量聚合酶链式反应（qRT-PCR）和蛋白质免疫印迹（Werstern Blot）检测细胞株中SMYD3（SET and MYND domain-containing protein 3）、VEGF（vascular endothelial growh factors）-C和VEGF-D mRNA和蛋白的表达。[结果] 胃癌MGC803细胞株中加入SMYD3抑制剂BCI-121后，淋巴管生成因子VEGF-C和VEGF-D mRNA和蛋白表达明显降低；在稳定低表达SMYD3的细胞株（MGC803-sh-SMYD3）中，发现较MGC80细胞株，MGC803-sh-SMYD3细胞株中VEGF-C和VEGF-D mRNA和蛋白明显降低。MTT检测不同时间节点不同浓度姜黄素作用MGC803细胞株细胞增殖的差异，发现50 μmol/Lol/L姜黄素对胃癌细胞抑制作用最强；姜黄素能够降低MGC803细胞株VEGF-C和VEGF-D mRNA和蛋白表达。BCI-121联合姜黄素处理MGC803细胞株后，较单独姜黄素处理，MGC803细胞株中VEGF-D mRNA和蛋白的表达降低，而VEGF-C无明显变化；进一步姜黄素处理MGC803和MGC803-sh-SMYD3细胞株后，同样发现MGC803-sh-SMYD3中VEGF-D mRNA和蛋白的表达降低，而VEGF-C变化不显著。[结论] 姜黄素可能通过抑制SMYD3通路进而抑制淋巴管生成相关因子VEGF-D。 |
| 关键词: 姜黄素 SMYD3 VEGF-C VEGF-D |
| DOI：10.11656/j.issn.1672-1519.2021.06.25 |
| 分类号:R735.2 |
| 基金项目:天津市教委科研计划项目（2018KJ015）。 |
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| Mechanism of anti-lymphangiogenesis related factors by curcumin in gastric cancer |
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LIU Honggen, YANG Peiying, LI Xiaojiang, JIA Yingjie
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Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the molecular mechanism of anti-lymphangiogenesis related factors by curcumin in gastric cancer.[Methods] The MTT was used to test the mobility of MGC803 cell lines. The SMYD3(SET and MYND domain-containing protein 3),VEGF(vascular endothelial growh factors)-C and VEGF-D mRNA and protein expression level were detected by quantitative real-time reverse transcription PCR and Werstern Blot.[Results] The expression of VEGF-C and VEGF-D mRNA and protein in MGC803 cell line were significantly lower after treating with SMYD3 inhibitor BCI-121 in MGC803 cell line. In low expression of SMYD3 cell line (MGC803-sh-SMYD3),the expression of VEGF-C and VEGF-D mRNA and protein were significantly lower than MGC803 cell line. MTT showed that 50 μmol/L curcumin had the strongest inhibitory effects,and the mRNA and protein expression levels of VEGF-C and VEGF-D were significantly eliminated by curcumin. Compared with treatment with curcumin alone,the expression of VEGF-D mRNA and protein were significantly decreased in MGC803 with treating both BCI-121 and curcumin. Furthermore,we found the expression of VEGF-D mRNA and protein in MGC803-sh-SMYD3 were decreased compared with MGC803 cell line when treated with curcumin,while not VEGF-C expression.[Conclusion] It suggested that curcumin might exert anti-VEGF-D in gastric cancer by inhibition of SMYD3 signaling. |
| Key words: curcumin SMYD3 VEGF-C VEGF-D |
