腹部推拿对大鼠乙醇性胃黏膜损伤的修复作用及机制研究

Healing effect and mechanism of abdominal massage on the ethanol-induced gastric mucosal injury in rats

关键词:  腹部推拿  乙醇性胃黏膜损伤  胃黏膜修复  机制研究

Key words:  abdominal massage  ethanol-induced gastric mucosal injury  gastric mucosal healing  mechanism research

摘要:

[目的] 研究腹部推拿对大鼠乙醇性胃黏膜损伤的修复作用及作用机制。[方法] 采用无水乙醇灌胃法复制胃黏膜损伤大鼠模型，将造模大鼠随机分为腹部推拿组和模型组，另设空白对照组。腹部推拿组每天予推拿干预，模型组和空白对照组不予任何干预手段，干预21 d后，观察大鼠行为学改变；取胃组织，观察大鼠胃黏膜组织损伤情况及病理改变；酶联免疫吸附（ELISA）法测定胃黏膜组织中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和表皮生长因子（EGF）的含量。[结果] 腹部推拿组大鼠扭体反应和旷场实验均有改善。模型组大鼠胃黏膜出现较严重的肉眼可见损伤，而经腹部推拿干预后胃黏膜损伤明显减轻。病理学结果显示，空白对照组大鼠胃黏膜结构完整，模型组大鼠胃黏膜损伤明显，出现炎性细胞浸润，而腹部推拿组大鼠胃黏膜损伤程度较轻。与空白对照组比较，模型组大鼠胃黏膜TNF-α、IL-6的分泌显著升高，EGF含量显著降低，经腹部推拿干预后炎性因子均有所下降，EGF显著升高。[结论] 腹部推拿可以通过降低胃黏膜TNF-α、IL-6的含量，提高EGF的含量，发挥促进大鼠乙醇性胃黏膜损伤的修复作用。

Abstract:

[Objective] To study the healing effect and mechanism of abdominal massage on the ethanol-induced gastric mucosal injury in rats.[Methods] The rat model of gastric mucosal injury was reproduced by absolute ethanol gavage. The rats were randomly divided into abdominal massage group and model group,and a blank control group was set up. The abdominal massage group was given massage intervention every day,and the model group and the blank control group were not given any intervention. After 21 days of intervention,the behavioral changes of rats were observed. The gastric tissues were taken to observe the damage and pathological changes of the gastric mucosal tissues of rats. The contents of TNF-α,IL-6,and EGF in the gastric mucosal tissues were determined by ELISA.[Results] The twisting reaction and open-field testof rats in the abdominal massage group were improved. The model control group had more serious visible damage to the gastric mucosa,while the damage to the gastric mucosa was reduced after the intervention of abdominal massage. The pathology showed that the gastric mucosa of rats in the blank control group was intact,and the gastric mucosa of rats in the model group was obviously damaged,with inflammatory cell infiltration,while the gastric mucosa of rats in the abdominal massage group was less damaged. Compared with the blank control group,the secretion of TNF-α and IL-6 in the model group was significantly increased,and the content of EGF was significantly decreased. After the intervention of abdominal massage,the inflammatory factors were decreased and EGF was significantly increased.[Conclusion] Abdominal massage can reduce the content of TNF-α and IL-6 in gastric mucosa,increase the content of EGF,and promote the healing of ethanol-induced gastric mucosal injury in rats.