| 摘要: |
| [目的] 探究美洲大蠊粪便提取物的抗氧化活性和化学成分。[方法] 采用1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除法测定美洲大蠊粪便提取物的抗氧化能力。综合利用溶剂提取法、大孔吸附树脂、制备高效液相色谱等分离纯化方法，对美洲大蠊粪便提取物中的化学成分进行提取分离，通过核磁共振波谱、质谱法鉴定化合物结构。[结果] DPPH法测试结果表明美洲大蠊粪便提取物具有明显的抗氧化活性，其半抑制浓度（IC50）为44.53 μg/mL。从中分离得到12个化合物，依据物理性质及波谱数据鉴定结构分别为：邻苯二酚（1）、苯甲酸（2）、羟吲哚（3）、5-羟基吲哚啉-2-酮（4）、8-羟基喹啉-2-羧酸（5）、6-羟基-2-喹啉酮（6）、8-羟基-2-喹啉酮（7）、2-喹啉酮（8）、6-羟基-3，4-二氢-2-喹啉酮（9）、5-羟基-3，4-二氢-2-喹啉酮（10）、8-羟基-3，4二氢-2-喹啉酮（11）、3，4-二氢-2-喹啉酮（12）。[结论] 美洲大蠊粪便提取物具有明显的抗氧化活性，分离得到的化合物主要为喹啉酮类化合物。 |
| 关键词: 美洲大蠊 肠道菌群 抗氧化活性 化学成分 |
| DOI：10.11656/j.issn.1672-1519.2021.08.23 |
| 分类号:R284.1 |
| 基金项目:天津科技计划项目（20ZYJDJC00070）。 |
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| Study on antioxidant activity and chemical components of excreta extracts from Periplaneta americana L. |
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WANG Ziyan, WU Dan, NIU Lu, WANG Yuefei, CHAI Xin
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Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| [Objective] To explore the antioxidant activity and chemical components of excreta extracts from Periplaneta americana L.[Methods] DPPH free radical scavenging method was used to investigate the antioxidant capacity of the excreta extracts from Periplaneta americana L. The chemical components were separated by using solvents extracting, macroporous resin and high performance liquid chromatography, and identified by mass and nuclear magnetic resonance spectrum.[Results] The half inhibitory concentration (IC50) of DPPH free radical scavenging assay was 44.53 μg/mL, which shows the obvious antioxidant activity of the excreta extracts from Periplaneta americana L. Twelve compounds isolated from the extracts were respectively identified as catechol(1), benzoic acid(2), oxindole (3), 5-hydroxyindolin-2-one (4), 8-hydroxy quinoline-2-carboxylic acid (5), 6-hydroxy-2(1H)-quinolinone, catechol (6), 8-hydroxy-2(1H)-quinolinone (7), quinolin-2(1H)-one (8), 6-hydroxy-3, 4-dihydro-1H-quinolin-2-one (9), 5-hydroxy-3, 4-dihydro-1H-quinolin-2-one (10), 8-hydroxy-3, 4-dihydro-1H-quinolin-2-one (11), and 3, 4-dihydro-1H-quinolin-2-one (12), based on the physical properties and spectral data.[Conclusion] The excreta extract of Periplaneta americana L. shows obvious antioxidant activity. The components isolated were mainly quinolones. |
| Key words: Periplaneta americana L. gut flora antioxidant activity chemical constituent |
