电针人迎、太冲穴对自发性高血压大鼠模型肾脏组织FoxP3和RORγt的影响

Effect of electroacupuncture at Renying and Taichong on kidney tissue Foxp3 and RORγt in spontaneous hypertension rats model

关键词:  针刺  高血压病前期  叉头状转录因子  维甲酸相关孤独核受体γt

Key words:  acupuncture  prehypertension  FoxP3  RORγt

摘要:

[目的] 观察“活血散风”针刺法对高血压前期大鼠肾脏组织叉头转录因子（FoxP3）和维甲酸相关孤独核受体（RORγt）蛋白和mRNA表达的影响。[方法] 将16只4周龄自发性高血压大鼠（SHRs）随机分为模型组（SHR组）和电针组（电针+SHR组），每组8只，另将8只同周龄WKY大鼠作为空白对照组（WKY组）。电针组给予针刺人迎、太冲治疗，每日1次，连续治疗2周。采用Softron无创血压仪测量干预前、干预1周、干预2周时的收缩压水平，苏木精-伊红（HE）染色观察大鼠肾脏结构形态，蛋白免疫印迹（Western blot）和聚合酶链反应（PCR）技术检测大鼠肾脏组织FoxP3、RORγt蛋白和mRNA表达情况。[结果] SHR大鼠收缩压水平明显高于WKY组（P<0.05），干预两周后，电针+SHR组收缩压水平明显低于SHR组（P<0.05）；SHR组肾脏组织RORγt mRNA表达明显高于WKY组（P<0.05），FoxP3蛋白和mRNA的表达、FoxP3/RORγt比值明显低于WKY组（P<0.05）；电针+SHR组肾脏组织RORγt mRNA表达明显低于SHR组（P<0.05），FoxP3蛋白表达、FoxP3/RORγt比值明显高于SHR组（P<0.05）；电针干预后SHR大鼠未出现明显的肾脏形态改变。[结论] “活血散风”针刺法可调节FoxP3和RORγt的表达，其对高血压前期大鼠的降压作用可能与调节FoxP3和RORγt的平衡有关。

Abstract:

[Objective] To observe the effect of "Huoxue Sanfeng" acupuncture on the protein and mRNA expression of FoxP3 and RORγt in kidney tissue of prehypertensive rats. [Method] Sixteen 4-week-old spontaneously hypertensive rats were randomly divided into a model group (SHR group) and an electro-acupuncture group (EA+SHR group),8 rats in each group. 8 WKY rats served as a blank control group. In EA+SHR group,acupuncture were performed at Renying and Taichong bilaterally,once a day for 2 weeks. The Softron non-invasive blood pressure measurement was used to measure the systolic blood pressure before intervention,1 week and 2 weeks after EA treatment. HE staining was used to detect the morphology of rats renal. Western blot and PCR technology were applied to assessed the FoxP3,RORγt protein and mRNA expression. [Result] The level of systolic blood pressure in SHR rats was significantly higher than that in WKY group (P<0.05). After two weeks of intervention,the level of systolic blood pressure in EA+SHR group was significantly lower than that of SHR group (P<0.05). The expression of RORγt mRNA in kidney tissue of SHR group was significantly higher than that of WKY group (P<0.05),FoxP3 protein and mRNA expression,as well as the FoxP3/RORγt ratio was significantly lower than that of WKY group (P<0.05). The renal tissue RORγt mRNA expression of EA+SHR group was significantly lower than that of SHR group (P<0.05),FoxP3 protein expression and FoxP3/RORγt ratio were significantly higher than that of SHR group (P<0.05). There was no obvious change of renal morphology after EA intervention. [Conclusion] "Huoxue Sanfeng" acupuncture can regulate the expression of FoxP3 and RORγt,and the antihypertensive effect of EA on prehypertensive rats may be related to regulating the balance of FoxP3 and RORγt.