解郁祛痰化浊方干预高脂饮食大鼠肠道菌群多样性的探讨

Study on the intervention of Jieyu Qutan Huazhuo Prescription on diversity of intestinal flora in rats with high fat diet

关键词:  肠道菌群  解郁祛痰化浊方  高脂血症  16S rDNA测序

Key words:  intestinal flora  Jieyu Qutan Huazhuo Prescription  hyperlipidemia  16S rDNA sequencing

摘要:

[目的] 探讨解郁祛痰化浊方干预高脂饮食大鼠肠道菌群的多样性及对血脂、体质量的影响。[方法] 将70只雄性SPF级别Wistar大鼠随机分为正常组，模型组，血脂康组，立普妥组，解郁祛痰化浊方低剂量组，中剂量组，高剂量组。每组10只。正常组喂养正常饲料，模型组喂养高脂饲料。连续灌胃给药8周后收集粪便，进行16S rRNA基因测序，测量体质量，腹主动脉取血检测胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）水平。[结果] 与正常组比较，模型组大鼠体质量明显增加（P<0.01）。与模型组比较，血脂康组，解郁祛痰化浊方低、高剂量组大鼠体质量明显下降（P<0.05）。模型组大鼠的血脂4项TC、TG、LDL-C均见明显升高，HDL-C降低（P<0.01）。同模型组比较，各用药组TC、TG均见显著降低，其中解郁祛痰化浊方中剂量组显著降低LDL-C水平（P<0.01），解郁祛痰化浊方高剂量组以及血脂康组可提高HDL-C水平。正常组厚壁菌门较模型组低（P<0.01），拟杆菌门较模型组高（P<0.01）。拟杆菌门解郁祛痰化浊方中剂量组较模型组增高（P<0.05）。厚壁菌门/拟杆菌门（F/B）模型组显著高于正常组（P<0.01），解郁祛痰化浊方中剂量组低于模型组（P<0.01）。[结论] 高脂血症以气郁痰阻为病机，解郁祛痰化浊方立方精准，调节高脂饮食大鼠血脂，降低体质量，调节F/B的比值及肠道菌群多样性。

Abstract:

[Objective] To explore the intervention of Jieyu Qutan Huazhuo Prescription on the diversity of intestinal flora and related serological indicators in blood fats and body weight. [Methods] Seventy male Wistar rats were randomly divided into normal group,model group,Xuezhikang group,Lipitor group,Jieyu Qutan Huazhuo Prescription low,medium and high dose group. 10 in each group. The normal group was fed with normal diet,and the model group was fed with high-fat diet. Stool was collected after continuous gavage for 8 weeks to detect the 16S rRNA gene sequencing,body mass was measured,and blood was taken from the abdominal aorta to detect the levels of cholesterol (TC),triglycerides (TG),low-density lipoprotein (LDL-C),and high-density lipoprotein (HDL-C). [Results] Compared with the normal group,the body weight of the model group increased significantly (P<0.01). Compared with the model group,the body weight of rats in the Xuezhikang group,low-dose Chinese medicine prescription group and high-dose Chinese medicine prescription group were significantly reduced (P<0.05).The TC,TG,LDL-C in the model group were significantly increased,and HDL-C were decreased (P<0.01).Compared with the model group,the TC and TG of each medication group were significantly reduced. Among them,the medium-dose Chinese medicine prescription group significantly reduced the LDL-C level (P<0.01),and the high-dose Chinese medicine group and the Xuezhikang group could increase the HDL-C level. Compared with the normal group,the UA of the model group was significantly increased (P<0.05). In the analysis of differences between the groups,compared with the normal group, the Desulfovibrio in the model group increased (P<0.01),and the difference was statistically significant. The Firmicutes in the normal group were lower than the model group (P<0.01),and the Bacteroides were higher than the model group (P<0.01). The medium-dose Chinese medicine prescription group in the Bacteroides were higher than that in the model group (P<0.05). The F/B model group was significantly higher than the normal group (P<0.01),and the medium-dose Chinese medicine prescription group were lower than the model group (P<0.01). [Conclusion] Hyperlipidemia is mostly caused by stagnation of qi and phlegm. Jieyu Qutan Huazhuo Prescription is precise,regulating blood lipids in rats with high-fat diet,reducing body weight,regulating F/B ratio and intestinal flora diversity,and protecting kidney function.