五味子多糖的分离、纯化及结构表征

Isolation,purification and characterization of polysaccharide from Schisandra chinensis

关键词:  五味子  多糖  分离纯化  结构表征

Key words:  Schisandra chinensis  polysaccharide  isolation and purification  structure identification

摘要:

[目的] 通过对五味子多糖的分离纯化、结构表征，以期为五味子的进一步开发利用提供科学研究数据。[方法] 采用水提醇沉法得五味子粗多糖，通过分级醇沉及葡聚糖凝胶G-150进一步分离纯化得五味子多糖组分。采用苯酚-硫酸法测定多糖的含量，高效分子排阻色谱联用多角度激光光散射检测器和示差检测器(HPSEC-MALLS-RID)的方法测定多糖的分子量，1-苯基-3-甲基-5-吡唑啉酮(PMP)试剂柱前衍生结合高效液相色谱(HPLC)法测定多糖的单糖组成，红外光谱(IR)和核磁共振波谱(NMR)分析多糖的结构。[结果] 分离纯化得五味子多糖组分SCP-3-1，其多糖含量为74.28%，分子量为84.9 kDa，主要由甘露糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖(摩尔比为0.09:0.19:19.82:2.08:0.13)组成。IR及NMR数据分析表明是一种以α-糖苷键连接的吡喃型多糖。[结论] 本研究对于进一步解析多糖结构具有一定的借鉴意义和参考价值，也为五味子的进一步应用提供了科学依据。

Abstract:

[Objective] The research on the isolation and characterization of polysaccharide from Schisandra chinensis is expected to provide scientific data for the further study of Schisandra chinensis.[Methods] The crude polysaccharides of Schisandra chinensis were obtained by water extraction and ethanol precipitation. A polysaccharide from Schisandra chinensis was further isolated and purified by graded ethanol precipitation and Sephadex G-150. Phenol-sulfuric acid method was used to determine the content of polysaccharide. High performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector method (HPSEC-MALLS-RID),high performance liquid chromatography (HPLC),infrared spectroscopy (IR) and nuclear magnetic resonance (NMR)were used to determine its molecular weight,monosaccharide compositions,functional groups and structure,respectively.[Results] A polysaccharide from Schisandra chinensis (SCP-3-1) was isolated from Schisandra chinensis. SCP-3-1 was mainly composed of mannose,galacturonic acid,glucose,galactose and arabinose in molar ratios of 0.09:0.19:19.82:2.08:0.13 with polysaccharide content of 74.28% and molecular weight of 84.9 kDa. IR and NMR analyses indicated that it was a pyran polysaccharide connected with α-glycosidic bonds.[Conclusion] This study can provide useful reference for further analyzing structure of polysaccharide,and also provide a scientific basis for the application of Schisandra chinensis.