慢肝养阴胶囊对酒精性肝炎大鼠肝组织Caspase-3及PTP1B表达的影响及相关性研究

Effects of Mangan Yangyin Capsule on the expression of Caspase-3 and PTP1B in liver tissues of alcoholic hepatitis rats and correlation study

关键词:  酒精性肝炎  慢肝养阴胶囊  氧化应激  凋亡  胰岛素抵抗

Key words:  alcoholic fatty liver  Mangan Yangyin Capsule  oxidative stress  apoptosis  insulin resistance

摘要:

[目的] 观察慢肝养阴胶囊对大量饮酒模型引起氧化应激、胰岛素抵抗和细胞凋亡等肝组织损伤的影响。[方法] 将50只SPF级Wistar大鼠按体质量随机分为空白对照组、模型对照组、慢肝养阴胶囊低剂量组（0.27 g/kg）、慢肝养阴胶囊高剂量组（0.54 g/kg）、多烯磷脂酰胆碱胶囊（阳性药物对照）组（0.15 mg/kg）。除空白对照组外其余各组用50％乙醇造模，灌胃体积为12 mL/kg。各给药组灌胃相应药物，10 mL/kg每日1次，空白对照组、模型对照组灌胃等体积的蒸馏水。边造模边给药16周。取静脉血，检测血清天冬氨酸氨基转移酶（AST）、γ-谷氨酰基转肽酶（γ-GT）。取肝组织1 g匀浆后提取上清液，检测肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）的活性。取肝左叶，用于苏木精-伊红（HE）染色，采用免疫组化法检测肝组织中活化半胱氨酸蛋白酶蛋白-3抗体（Caspase-3）和蛋白酪氨酸磷酸酶B1抗体（PTP1B）的表达。[结果] 慢肝养阴胶囊可显著降低血清中AST、γ-GT活性（P<0.05或P<0.01），显著增加肝组织中SOD活性（P<0.05或P<0.01），显著降低肝组织中MDA含量（P<0.05或P<0.01），显著降低Caspase-3和PTP1B在肝组织中的表达（P<0.05或P<0.01）。慢肝养阴胶囊各治疗组在肝组织中存在一定数量的脂肪空泡，但脂肪空泡和炎细胞浸润较模型对照组有一定的减轻。[结论] 慢肝养阴胶囊可能通过提高机体的抗氧化能力、减轻胰岛素抵抗、抗凋亡等途径对大量饮酒产生的肝损伤起到保护作用。

Abstract:

[Objective] To observe the effect of Mangan Yangyin Capsules on rat alcoholic hepatitis model.[Methods] The 50 wistar rats were randomly divided into blank control group,model control group,and Mangan Yangyin Capsules(0.27 g/kg low-dose group,0.54 g/kg high-dose group). Polyene phosphatidylcholine capsule positive drug control group(0.15 mg/kg),except the blank control group,the other groups were intragastrically administered with 50% ethanol at 12 mL/kg. Each administration group was intragastrically administered with corresponding drugs,once a day,10 mL/kg,the blank control group and model control group were intragastrically administered with equal volume of distilled water. The drug was administered for 16 weeks while building the model. Take venous blood and test serum AST and γ-GT. After homogenizing 1g of liver tissue,extract the supernatant to detect the content of SOD and MDA in liver tissue. The remaining liver tissue was used for HE staining,and the expression of Caspase-3 and PTP1B in the liver tissue was detected by immunohistochemistry.[Results] Mangan Yangyin Capsules can significantly reduce the activity of AST and γ-GT in serum (P<0.05 or P<0.01). Mangan Yangyin Capsules can significantly increase the SOD activity in liver tissues (P<0.05 or P<0.01). Mangan Yangyin Capsules can significantly reduce the content of MDA in liver tissue (P<0.05 or P<0.01). Mangan Yangyin Capsules can significantly reduce the expression of Caspase-3 and PTP1B in liver tissues (P<0.05 or P<0.01). There were a certain number of fatty vacuoles in the liver tissues of Mangan Yangyin capsules in each treatment group,but fatty vacuoles and inflammatory cell infiltration were reduced to a certain extent compared with the model control group.[Conclusion] Mangan Yangyin Capsules may protect liver injury caused by heavy drinking by improving the body's antioxidant capacity,reducing insulin resistance,and anti-apoptosis.